您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An indirect competitive ELISA for determination of guanidine acetic acid in animal feed

文献类型： 外文期刊

作者： Yang, Xingdong 1 ; Li, Lili 1 ; Qu, Yang 1 ; Wu, Lihua 1 ; Wang, Chenchen 1 ; He, Beibei 1 ; Zang, Zhenchun 1 ; Hu, Xiaofei 2 ;

作者机构： 1.Zhoukou Normal Univ, Coll Life Sci & Agron, Zhoukou 466001, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

关键词： Guanidino acetic acid; Immunogen; Monoclonal antibody; ELISA; Animal feed

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： To establish an indirect competitive ELISA (ci-ELISA) for the detection of guanidino acetic acid (GAA) residues in animal feed, in this study, GAA was coupled to carrier proteins via the active ester method to obtain an anti-GAA complete antigen (GAA-BSA) and a detection antigen (GAA-OVA). BALB/c mice were immunized with GAA-BSA, after which anti-GAA monoclonal antibodies were prepared via hybridoma and other techniques. An ic-ELISA method was developed by optimizing the reaction conditions and the accuracy, precision and specificity of the method were determined. The results showed that GAA was successfully coupled to the carrier protein; a hybridoma cell line (2C4) against GAA was obtained, and the IC50 value of the monoclonal antibody was 4.65 mu g/kg; The average recovery rate of GAA spiked in animal feed by this method was 87.4%, and its intra-assay coefficients of variation were greater than the inter-assay coefficients of variation in all assays; no cross-reaction with the other competing reactants was detected. The indirect competitive ELISA method developed in this study was able to fulfil the requirements for the determination of GAA r esidues in animal feed.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台