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Establishment of a Chemiluminescent ELISA Method for Florfenicol in Eggs and Chicken Meat

文献类型： 外文期刊

作者： Ge, Mengjun 1 ; Xing, Yunrui 2 ; Sun, Yaning 2 ; Cai, Wenjin 1 ; Wang, Yao 1 ; Hu, Xiaofei 2 ;

作者机构： 1.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471000, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Hlth, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

关键词： Florfenicol; Hazard; Ci-ELISA; Egg; Chicken meat

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.0； 五年影响因子：3.1 ）

ISSN： 1936-9751

年卷期： 2024 年

页码：

收录情况： SCI

摘要： To establish a highly sensitive chemiluminescent enzyme-linked immunosorbent assay (ci-ELISA) method for the determination of florfenicol (FF) in egg and chicken meat. Based on the obtained monoclonal antibodies against florfenicol, a chessboard test was employed to determine the optimal working concentration of artificial antigen and antibody, and then established a ci-ELISA method, and characterized its performance. The results showed that the ci-ELISA method had a detection limit of 5.433 pg/mL, an IC50 of 30.893 pg/mL, and a detection range between 5.433 and 193.577 pg/mL. The coefficient of variation was below 10%, indicating satisfactory precision. The spiked recoveries in egg samples were between 90.03% and 95.24%, and the spiked recoveries in chicken meat samples were between 90.56% and 93.04%. In conclusion, the developed ci-ELISA method is highly sensitive and suitable for monitoring and detection of trace amounts of FF residues in eggs and chicken meat.

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