A highly sensitive and specific immunochromatographic test strip for detecting p,p′-DDA in seawater and shellfish
文献类型： 外文期刊
作者： Zhang, Teng 1 ; Xing, Yunrui 2 ; Yao, Jiale 1 ; Yang, Zhuan 1 ; Wang, Jucai 3 ; Chen, Linlin 4 ; Yao, Lunguang 1 ;
作者机构： 1.Nanyang Normal Univ, Henan Prov Engn & Technol Ctr Hlth Prod Livestock, Sch Life Sci & Agr Engn, Nanyang 473000, Peoples R China
2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
3.Luoyang Normal Univ, Coll Food & Drug, Luoyang 471934, Peoples R China
4.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450046, Peoples R China
关键词： p ' -DDA; Monoclonal antibody; Recombinant full-length antibody; Immunochromatographic strip; Recognition mechanism
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 492 卷
页码：
收录情况： SCI
摘要： P,p '-DDA, similar to DDT, accumulates in the food chain and poses potential risks to human health. Herein, a novel sensitive and specific mAb-10B4D7 was screened and subsequently rAb-10B4D7 was expressed by genetic engineering. Utilizing rAb-10B4D7, an immunochromatographic test strip (ITS) was developed with visual limit of detection 12.5 mu g/L within 15 min, displaying no cross-reactivity with o,p '-DDT, p,p '-DDT, p,p '-DDD, and p,p ' DDE. Further, a linear relationship between log(concentration of p,p '-DDA) and the optical density ratio of test (T) line and control (C) line was established with limits of detection of 1.03 mu g/kg, recoveries of 79.3-106.8 %, and coefficients of variation below 10 %. Molecular docking simulations identified two critical residues (ArgH62, Phe-L98) forming three hydrogen bonds with p,p '-DDA, resulting in the high specificity and affinity of rAb10B4D7, with a KD of 5.81 x 10-9 mol/L. These results indicated that the ITS was suitable for monitoring p,p ' DDA and organochlorine residues in seawater and shellfish.
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