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A novel metal-organic framework based electrochemical immunosensor for the rapid detection of Salmonella typhimurium detection in milk

文献类型： 外文期刊

作者： Zhan, Ke 1 ; Chen, Linlin 1 ; Li, Shanshan 2 ; Yu, Qiuying 1 ; Zhao, Zheng 1 ; Li, Junwei 1 ; Xing, Yunrui 6 ; Ren, Hongtao 1 ; Wang, Na 2 ; Zhang, Gaiping 3 ;

作者机构： 1.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Henan, Peoples R China

2.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China

3.Coll Vet Med Int Joint Res Ctr Anim Immunol, Zhengzhou 450046, Henan, Peoples R China

4.Longhu Lab Adv Immunol, Zhengzhou 450046, Peoples R China

5.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China

6.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China

7.Key Lab Nutr & Hlth Food Zhengzhou, Zhengzhou 450002, Henan, Peoples R China

关键词： Salmonella typhimurium; Electrochemical immunosensor; Food safety; Metal -organic framework; Platinum nanoparticles; Multiwalled carbon nanotubes

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 444 卷

页码：

收录情况： SCI

摘要： Salmonella is one of the most prevalent pathogens causing foodborne diseases. In this study, a novel electrochemical immunosensor was designed for the rapid and accurate detection of Salmonella typhimurium (S. typhimurium) in milk. Platinum nanoparticles and Co/Zn-metal-organic framework @carboxylic multiwalled carbon nanotubes in the immunosensor acted synergistically to enhance the sensing sensitivity and stability. The materials and sensors were characterised using X-ray diffractometry, scanning electron microscopy, Fouriertransform infrared spectroscopy, differential pulse voltammetry, cyclic voltammetry, and other techniques. The optimised immunosensor showed a linear response for S. typhimurium concentrations in the range from 1.3 x 102 to 1.3 x 108 CFU mL-1, with a detection limit of 9.4 x 101 CFU mL-1. The assay also demonstrates good specificity, reproducibility, stability, and practical application potential, and the method can be extended to other foodborne pathogens.

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