Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV
文献类型： 外文期刊
作者： Yang, Suzhen 1 ; Sun, Yaning 1 ; Xing, Yunrui 1 ; Fan, Lu 1 ; Li, Yongliang 2 ; Shang, Yanli 1 ; Chai, Shujun 1 ; Liu, Yunchao 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Anim Hlth, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Anhui Divin Biol Prod Co Ltd, Bozhou 236800, Anhui, Peoples R China
关键词： PRV; gB; Time-resolved fluorescence immunochromatographic strip; ELISA
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）
ISSN：
年卷期： 2025 年 21 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundPseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and straight forward method to monitor PRV-induced antibody levels in practice.ResultsA time-resolved fluorescence immunochromatographic (TRFIC) strip was developed for the serological detection of PRV gB antibodies in swine. Following systematic analysis and evaluation, the assay demonstrated a high degree of correlation with established reference method. The positive and negative coincidence rates between the TRFIC strip and ELISA were 96.8% and 94.2%, respectively. Furthermore, comprehensive analytical and comparative assessments revealed that the TRFIC strip exhibited no cross-reactivity with antibodies against other porcine pathogens.ConclusionGiven its high specificity, sensitivity, and convenience, the TRFIC strip is suitable for on-site detection of PRV gB antibodies and can serve as a valuable tool for monitoring PRV immune status in animal populations.
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