Integrative transcriptomics and proteomics analysis provide a deep insight into goose astrovirus-host interactions during GAstV infection
文献类型： 外文期刊
作者： Shi, Jianzhou 1 ; Jin, Qianyue 3 ; Zhao, Jinbing 1 ; Yu, Jinran 1 ; Yu, Xianyi 1 ; Sun, Guirong 2 ; Yao, Lunguang 1 ;
作者机构： 1.Nanyang Normal Univ, Sch Life Sci, Nanyang 473061, Henan, Peoples R China
2.Shennong Lab, Zhengzhou 450046, Henan, Peoples R China
3.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China
4.Henan Agr Univ, Anim Sci & Technol, Zhengzhou 450046, Henan, Peoples R China
5.Cent Route South to North Water Divers Project, Henan Field Observat & Res Stn Headwork Wetland Ec, Nanyang 473061, Henan, Peoples R China
6.Henan Prov Engn & Technol Ctr Hlth Prod Livestock, Nanyang 473061, Henan, Peoples R China
关键词： goose astrovirus; transcriptome; proteome
期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）
ISSN： 0032-5791
年卷期： 2024 年 103 卷 12 期
页码：
收录情况： SCI
摘要： Goose astrovirus (GAstV) is a newly discovered astrovirus. GAstV causes gout and death in 4- to-16-day-old goslings. For the past few years, fatal gout, the cardinal clinical symptom of gosling infected with GAstV, has been spreading rapidly in some goose Chinese farms, which caused continuous economic losses to the goose breeding industry in China. Currently, several underlying mechanisms involved in viral replication, inflammatory reaction, virions release, and viral pathogenesis of GAstV remain to be elucidated. In this study, we explored the mechanisms of GAstV-host interactions, the transcriptome and proteome profiles of GAstV-infected LMH cells were sequenced by RNA-seq and data-independent acquisition (DIA) techniques, respectively, and followed using an integrative analysis. Compared with uninfected LMH cells, a total of 322 differentially expressed genes (DEG) (195 up-regulated, 127 down-regulated) and 36 differentially expressed proteins (DEP) (31 up-regulated, 5 down-regulated) were detected. Nine DEGs were randomly selected for further validation by quantitative real-time polymerase chain reaction (qPCR). Through GO and KEGG enrichment analysis, DEG and DEP were significantly enriched in several important cellular signaling pathways, including MAPK, PI3K-Akt, cAMP, chemokine, calcium, phospholipase D, Ras, TNF, IL-17, Rap1, NF-kappa B signaling pathways, indicating that GAstV affects cell growth and immune signaling. This study provided an overview of changes in transcriptome and proteome profiles of GAstV-infected LMH cells, therefore, providing a crucial basis to further explore the mechanisms of GAstV-host interactions.
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