Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration
文献类型： 外文期刊
作者： Liu, Xin-Xin 1 ; Wang, Yao 1 ; Zhang, Jian-Hui 3 ; Lu, Yun-Feng 1 ; Dong, Zi-Xing 1 ; Yue, Chao 1 ; Huang, Xian-Qing 4 ; Zhang, Si-Pu 5 ; Li, Dan-Dan 1 ; Yao, Lun-Guang 1 ; Tang, Cun-Duo 1 ;
作者机构： 1.Nanyang Normal Univ, Henan Prov Engn Lab Insect Bioreactor, Henan Int Joint Lab Insect Biol, 1638 Wolong Rd, Nanyang 473061, Henan, Peoples R China
2.Nanyang Normal Univ, Henan Key Lab Insect Biol Funiu Mt, 1638 Wolong Rd, Nanyang 473061, Henan, Peoples R China
3.She Dian Lao Jiu Co Ltd, Postdoctoral Innovat Practice Base, 2 Liquor Ave, Nanyang 473300, Henan, Peoples R China
4.Henan Agr Univ, Coll Food Sci & Technol, 63 Agr Rd, Zhengzhou 450002, Henan, Peoples R China
5.Henan Acad Agr Sci, Zhengzhou 450002, Peoples R China
关键词： 2,5-dimethylpyrazine; Metabolic engineering; Microbial cell factories; Aminoacetone oxidase; Threonine transporter; Whole-cell catalysis
期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
ISSN：
年卷期： 2024 年 17 卷 1 期
页码：
收录情况： SCI
摘要： 2,5-Dimethylpyrazine (2,5-DMP) is important pharmaceutical raw material and food flavoring agent. Recently, engineering microbes to produce 2,5-DMP has become an attractive alternative to chemical synthesis approach. In this study, metabolic engineering strategies were used to optimize the modified Escherichia coli BL21 (DE3) strain for efficient synthesis of 2,5-DMP using L-threonine dehydrogenase (EcTDH) from Escherichia coli BL21, NADH oxidase (EhNOX) from Enterococcus hirae, aminoacetone oxidase (ScAAO) from Streptococcus cristatus and L-threonine transporter protein (EcSstT) from Escherichia coli BL21, respectively. We further optimized the reaction conditions for synthesizing 2,5-DMP. In optimized conditions, the modified strain can convert L-threonine to obtain 2,5-DMP with a yield of 2897.30 mg/L. Therefore, the strategies used in this study contribute to the development of high-level cell factories for 2,5-DMP.
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