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An accurate and amplifying competitive lateral flow immunoassay for the sensitive detection of haptens

文献类型： 外文期刊

作者： Sun, Yaning 1 ; Yang, Suzhen 1 ; Yang, Jifei 1 ; Hu, Xiaofei 1 ; Wei, Qiang 1 ; Wang, Yao 3 ; Xing, Yunrui 1 ; Chen, Linl 1 ;

作者机构： 1.Minist Agr, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Henan Acad Agr Sci, Zhengzhou, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China

3.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang, Peoples R China

关键词： Sensitive enhancement; competitive lateral flow immunoassay; test strip; GM; 2; 4-d

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2021 年 32 卷 1 期

页码：

收录情况： SCI

摘要： We report a new competitive lateral flow immunoassay (LFIA) for hapten detection, which uses antibodies to bind antigens from a sample and form a coloured immune complex due to artificial antigens conjugated with gold nanoparticles; these were intercepted with SPA. Here, 2,4-dichlorophenoxyacetic acid (2,4-D) and gentamicin (GM) were selected as model analytes. The visual limit of detection of the 2,4-D strip was 25 ng/mL in tap water; the GM strip was 3.13 ng/mL in milk. This approach exhibits more than a 40-fold lower detection limit for 2,4-D and 3.2-fold lower for GM, which is compared to traditional LFIA using the same reagents. This approach can accurately control the amount of antibody, protect the activity of antibody and enhance the detection signal, thus improving the sensitivity of the LFIA and providing more and more sensitive methods for the development of LFIAs.

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