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Electrochemical immunosensor based on MOF for rapid detection of 6-benzyladenine in bean sprouts

文献类型： 外文期刊

作者： Liu, Qingqing 1 ; Xing, Yunrui 1 ; Pang, Xinghao 1 ; Zhan, Ke 2 ; Sun, Yaning 1 ; Wang, Na 1 ; Hu, Xiaofei 1 ;

作者机构： 1.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Henan 450002, Peoples R China

2.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Henan, Peoples R China

3.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China

4.Int Joint Res Ctr Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

5.Key Lab Nutr & Hlth Food Zhengzhou, Zhengzhou 450002, Peoples R China

6.Henan Acad Agr Sci, Key Lab Anim Immunol Minist Agr, Zhengzhou 450002, Peoples R China

关键词： Electrochemical immunosensor; Food safety; AuNPs; Zn; Ni-ZIF-8-800 @graphene; Rapid detection; 6-benzylaminopurine; Bean sprouts

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）

ISSN： 0889-1575

年卷期： 2023 年 115 卷

页码：

收录情况： SCI

摘要： 6-benzylaminopurine (6-BA) is a widely used plant growth promoter. However, it can lead to precocious puberty and cause acute poisoning and even cancer of consumer. Thus, it is necessary to establish rapid and sensitive method for the detection of 6-BA. Electrochemical immunosensor is a new kind of biosensor, which combines the high selectivity of antibody with the high sensitivity of electrochemical sensor. In this study, an electrochemical immunosensor was developed for the detection of 6-BA in bean sprouts. The carbon material of Zn/Ni-ZIF-8-800 derived from bimetal-organic framework was synthesized by solvothermal method. Then, Zn/Ni-ZIF-8-800 was ultrasonically mixed with the highly conductive graphene, and the gold nanoparticles (AuNPs) were reduced by constant potential deposition (AuNPs/Zn/Ni-ZIF-8-800 @graphene). Finally, the antibody of 6-BA was combined with the prepared material to make an electrochemical immunosensor. The prepared materials were charac-terized by XRD, FTIR, SEM, TEM and XPS analyses. In addition, the electron transfer abilities of the modified sensors were investigated by CV and EIS. The established immunosensor presented a wide linear range (0.08-50 ng/mL), low detection limitation (0.24 ng/mL) and satisfactory recovery rates towards 6-BA (90.9-105.0 %). In brief, an electrochemical immunosensor with high sensitivity and selectivity was developed, which could be used to detect 6-BA in bean sprouts effectively.

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