A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops
文献类型： 外文期刊
作者： Yang, Qianwen 1 ; Wang, Yu 1 ; Liu, Xiaofeng 2 ; Liu, Hua 1 ; Bao, Huifang 4 ; Wang, Jinbin 1 ; Zeng, Haijuan 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China
3.Minist Agr & Rural Affairs, Crops Ecol Environm Secur Inspect & Supervis Ctr, Shanghai, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Urumqi, Peoples R China
关键词： immunosensor; thionine; gold nanoparticles; label free; genetically modified crops
期刊名称：FRONTIERS IN CHEMISTRY （ 影响因子：5.545； 五年影响因子：5.869 ）
ISSN： 2296-2646
年卷期： 2021 年 9 卷
页码：
收录情况： SCI
摘要： Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for the ultrasensitive detection of PAT protein was constructed using thionine (Thi)/gold nanoparticles (AuNPs) as signal amplification molecules and electrochemically active substances. Under optimum conditions, the limits of detection of the sensor for soybean A2704-12 and maize BT-176 were 0.02% and 0.03%, respectively. The sensor could detect crops containing PAT protein and had no cross-reaction with other proteins. After storage at 4 degrees C for 33 days, the sensor still retained 82.5% of the original signal, with a relative standard deviation (RSD) of 0.92%. The recoveries of the sensor for soybean A2704-12 and maize BT-176 were 85%-108% and 98%-113%, respectively. The developed PAT-target immunosensor with high sensitivity, specificity, and satisfactory reproducibility and accuracy will be a useful tool in the trace screening of GM crops. Moreover, this design concept can be extended to other proteins by simply changing the antibody.
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