文献类型： 外文期刊

作者： Luo, Jing ¹ ; Huang, Sha ¹ ; Wang, Min ² ; Zhang, Ruimao ⁴ ; Zhao, Degang ³ ; Yang, Yuanyu ¹ ; Wang, Fang ¹ ; Wang, Zhuanzhuan ¹ ; Tang, Rong ¹ ; Wang, Lulu ¹ ; Xiao, Huagui ¹ ; Yang, Bin ¹ ; Li, Chao ¹ ;

作者机构： 1.Guizhou Acad Agr Sci, Guizhou Oil Crops Inst, 502 Xinzhong Rd, Guiyang 550006, Peoples R China

2.Guizhou Normal Univ, Huaxi Univ City, Sch Life Sci, Guiyang 550025, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Plant Conservat Technol Ctr, Jinxin Community, 502 Xinzhong Rd, Guiyang 550006, Peoples R China

4.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, 111 Duyun Rd, Guiyang 520115, Peoples R China

5.Guizhou Univ, Coll Life Sci, Collaborat Innovat Ctr Mt Ecol & Agrobioengn, Inst Agrobioengn, 2708 Huaxi Ave South Sect, Guiyang 550025, Peoples R China

6.Guizhou Univ, Coll Life Sci, Inst Agrobioengn,Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inn, 2708 Huaxi Ave South Sect, Guiyang 550025, Peoples R China

关键词： Brassica napus L.; dwarfing mechanism; multiomics; plant height

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dwarfing mechanism of DW871 and provide a theoretical foundation for future applications of the variety, we used transcriptome and proteome sequencing to identify genes and proteins associated with the dwarfing phenotype, using homologous high-stalk material HW871 as a control. By RNA-seq and iTRAQ, we discovered 8665 DEGs and 50 DAPs. Comprehensive transcription and translation level analysis revealed 25 correlations, 23 of which have the same expression trend, involving monolignin synthesis, pectin-lignin assembly, lignification, glucose modification, cell wall composition and architecture, cell morphology, vascular bundle development, and stalk tissue composition and architecture. As a result of these results, we can formulate a hypothesis about the DW871 dwarfing phenotype: plant hormone signal transduction, such as IAA and BRs, is linked to the formation of dwarf phenotypes, and metabolic pathways related to lignin synthesis, such as phenylpropane biosynthesis, also play a role. Our works will contribute to a better understanding of the genes and proteins involved in the rapeseed dwarf phenotype, and we will propose new insights into the dwarfing mechanism of Brassica napus L.