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Transcriptomic analysis of the antioxidant responses and immunomodulatory effects of dietary melatonin in red swamp crayfish (Procambarus clarkii)

文献类型： 外文期刊

作者： Li, Yiming 1 ; Ye, Yucong 2 ; Li, Siwen 2 ; Feng, Jianbin 4 ; Liu, Xingguo 1 ; Che, Xuan 1 ; Jiang, Qichen 3 ; Chen, Xiaolong 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Fishery Machinery & Instrument Res Inst, Shanghai 200092, Peoples R China

2.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

3.Freshwater Fisheries Res Inst Jiangsu Prov, Nanjing 210017, Peoples R China

4.Shanghai Ocean Univ, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China

5.Chinese Acad Fisheries Sci, Fishery Machinery & Instrument Res Inst, 63 Chifeng Rd, Shanghai 200092, Peoples R China

关键词： Melatonin; Transcriptome analysis; Immunomodulatory; Procambarus clarkia

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 142 卷

页码：

收录情况： SCI

摘要： This study aimed to investigate the effects of dietary melatonin (MT) levels on the antioxidant capacity, immunomodulatory, and transcriptional regulation of red swamp crayfish. Six experimental diets with different levels of MT (0, 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg diet) were fed to juvenile crayfish for 60 d. The transcriptome data of the control group and the group supplemented with dietary MT at 165.1 mg/kg were obtained using RNA-seq. In total, 3653 differentially expressed genes (2082 up-regulated and 1571 down regulated) were identified. Pathways and genes related to antioxidant immune and growth performance were verified by qRT-PCR. The total hemocyte count, phagocytosis rate, and respiratory burst were significantly increased in the MT (165.1 mg/kg) group compared to the control group. Analysis of antioxidant immune-related enzymes in the hepatopancreas demonstrated that dietary MT (165.1 mg/kg) significantly increased activities of catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase and significantly decreased aspartate aminotransferase and alanine aminotransferase activity. At the transcriptional level, dietary MT up regulated expression levels of genes associated with antioxidant immune and development, which included toll-like receptors, Crustin, C-type lectin, and so on. To conclude, MT could be used as a supplement in crayfish feed to increase immunity and antioxidant capacity and according to the broken line regression, the ideal MT concentration was the 159.02 mg/kg. Overall, this study demonstrates the role of melatonin in the antioxidant responses and immunomodulatory of Procambarus clarkii, laying the foundation for the development of melatonin as a feed additive in the aquaculture of this species.

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