文献类型： 外文期刊

作者： Yi, Yuanyang ¹ ; Wang, Yuxian ² ; Liu, Wanqin ² ; Zhu, Jing ³ ; Gu, Meiying ³ ; Jia, Qiong ³ ; Li, Xue ⁴ ; Mutalifu, Munire ⁴ ; Jiang, Ling ⁵ ; Zhang, Wei ¹ ; Zhang, Zhidong ¹ ;

作者机构： 1.Xinjiang Normal Univ, Coll Life Sci, Urumqi, Peoples R China

2.Xinjiang Univ, Coll Life Sci & Technol, Urumqi, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Key Lab Special Environm Microbiol, Urumqi 830091, Peoples R China

4.Xinjiang Agr Univ, Coll Food Sci & Pharmaceut Sci, Urumqi, Peoples R China

5.Nanjing Tech Univ, Coll Food Sci & Light Ind, State Key Lab Mat Oriented Chem Engn, Nanjing, Peoples R China

关键词： Di-n-butyl phthalate; biodegradation; whole-genome sequencing; metabolomics analysis; Priestia megaterium P-7

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Introduction Di-n-butyl phthalate (DBP) is one of the most widely used phthalate esters (PAEs) and is considered an emerging global pollutant. It may pose a significant threat to ecosystem and human health due to its residual hazards and accumulation in the environment. Bacteria-driven PAE biodegradation is considered an economical and effective strategy for remediating such polluted environments.Methods A DBP-degrading bacterium (P-7), was isolated from long-term film mulched cotton field soil. Its identity was confirmed via physiological, biochemical, and 16S rRNA gene analyses. The degradation conditions were optimized through single-factor experiments and response surface methodology (RSM).Furthermore, the whole-genome sequencing coupled with metabolomics was employed to elucidate metabolic mechanisms.Results Priestia megaterium P-7 (P. megaterium P-7) achieved 100% DBP removal within 20 h under optimal conditions and exhibited broad substrate specificity for other PAEs. Genomic analysis identified key genes (lip, aes, ybfF, estA, and yvaK) encoding esterases/hydrolases that initiate DBP catabolism, converting it to phthalic acid (PA). Subsequent decarboxylation (pdc, bsdCD, mdcACDH, and lysA) and dioxygenase-mediated steps integrated PA into the TCA cycle. Metabolomics revealed three degradation pathways: decarboxylation (DBP -> MBP -> BB -> BA -> Catechol), hydrolysis (DBP -> MBP -> PA -> PCA -> Catechol) and direct beta-oxidation (DBP -> DEP -> MEP -> PA -> Catechol).Conclusion P. megaterium P-7 demonstrates exceptional degradation efficiency, substrate versatility, and environmental stress tolerance, making it a promising candidate for bioremediation of organic pollutants in contaminated soil.