The genetic analysis and germplasm identification of the gametophytes of Undaria pinnatifida (Phaeophyceae) with RAPD method
文献类型: 外文期刊
作者: Wang, Di 1 ; Wang, Xiuliang 2 ; Li, Dapeng 2 ; Wang, Feijiu 2 ; Duan, Delin 2 ;
作者机构: 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China; Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词: DNA;Gametophytes;Genetic analysis;Genetic DNA;Gametophytes;Genetic analysis;Genetic distance;Germplasm;Lesions;Nucleotide sequence;Polymerase chain reaction;Random amplified polymorphic DNA;Screening;Taxonomy;Laminaria;Phaeophyceae;Undaria;Undaria pinnatifida
期刊名称:JOURNAL OF APPLIED PHYCOLOGY ( 影响因子:3.215; 五年影响因子:3.612 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Eleven pairs of Undaria pinnatifida (Harv.) Suringar gametophytes were identified with random amplified polymorphic DNA (RAPD) technique. After screening 100 primers, 20 ten-base primers were determined for the RAPD analysis. A total of 312 polymorphic loci were obtained, of which 97.7% were polymorphic. The primer S198 was found to distinguish all the selected Undaria pinnatifida gametophytes. The genetic distances between each two of the twenty-two U. pinnatifida gametophytes ranged from 0.080 to 0.428, while the distances to the Laminaria was 0.497 on average. After reexamination, two sequences characterized amplification region (SCAR) markers were successfully converted, which could be applied to U. pinnatifida germplasm identification. All these results demonstrated the feasibility of applying RAPD markers to germplasm characterization and identification of U. pinnatifida gametophytes, and to provide a molecular basis for Undaria breeding.
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