Puerarin attenuates isoproterenol-induced myocardial hypertrophy via inhibition of the Wnt/beta-catenin signaling pathway
文献类型： 外文期刊
作者： Wang, Xiaoying 1 ; He, Kai 1 ; Ma, Linlin 2 ; Wu, Lan 2 ; Yang, Yan 3 ; Li, Yanfei 1 ;
作者机构： 1.Shanghai Univ Tradit Chinese Med, Grad Sch, Shanghai 201203, Peoples R China
2.Shanghai Univ Med & Hlth Sci, Coll Med Technol, Shanghai 201318, Peoples R China
3.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
4.Shanghai Acad Agr Sci, Inst Edible Fungi, Bldg 12,1000 Jinqi Rd, Shanghai 201106, Peoples R China
5.Shanghai Univ Med & Hlth Sci, Coll Med Technol, 279 Zhouzhu Rd, Shanghai 201318, Peoples R China
关键词： puerarin; isoproterenol; cardiac hypertrophy; Wnt; beta-catenin signaling pathway; phosphorylation of NF-kappa B; p65
期刊名称：MOLECULAR MEDICINE REPORTS （ 影响因子：3.423； 五年影响因子：3.112 ）
ISSN： 1791-2997
年卷期： 2022 年 26 卷 4 期
页码：
收录情况： SCI
摘要： Myocardial hypertrophy (MH) is an independent risk factor for cardiovascular disease, which in turn lead to arrhythmia or heart failure. Therefore, attention must be paid to formulation of therapeutic strategies for MH. Puerarin is a key bioactive ingredient isolated from Pueraria genera of plants that is beneficial for the treatment of MH. However, its molecular mechanism of action has not been fully determined. In the present study, 40 mu M puerarin was demonstrated to be a safe dose for human AC16 cells using Cell Counting Kit-8 assay. The protective effects of puerarin against MH were demonstrated in AC16 cells stimulated with isoproterenol (ISO). These effects were characterized by a significant decrease in surface area of cells (assessed using fluorescence staining) and mRNA and protein expression levels of MH-associated biomarkers, including atrial and brain natriuretic peptide, assessed using reverse transcription-quantitative PCR and western blotting, as well as beta-myosin heavy chain mRNA expression levels. Mechanistically, western blotting demonstrated that puerarin inhibited activation of the Wnt signaling pathway. Puerarin also significantly decreased phosphorylation of p65; this was mediated via crosstalk between the Wnt and NF-kappa B signaling pathways. An inhibitor (Dickkopf-1) and activator (IM-12) of the Wnt signaling pathway were used to demonstrate that puerarin-mediated effects alleviated ISO-induced MH via the Wnt signaling pathway. The results of the present study demonstrated that puerarin pre-treatment may be a potential therapeutic strategy for preventing ISO-induced MH and managing MH in the future.
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