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Development and characterization of monoclonal antibody against the critical loop structure of african swine fever virus P72 protein

文献类型： 外文期刊

作者： Chang, Zejie 1 ; Du, Yongkun 1 ; Li, Ruiqi 2 ; Sun, Xueke 2 ; Chen, Yilan 2 ; Li, Minghui 1 ; Fan, Lu 2 ; Liu, Siyuan 1 ; Wang, Siqiao 2 ; Ding, Peiyang 3 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Agr Univ, Coll Anim Med, Zhengzhou 450046, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China

4.Longhu Adv Immunizat Lab, Zhengzhou 450046, Peoples R China

5.Peking Univ, Inst Adv Agr Sci, Beijing 100080, Peoples R China

6.Zhengzhou Univ, Coll Life Sci, Zhengzhou 450001, Peoples R China

7.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China

关键词： African swine fever virus; P72 protein; Monoclonal antibody; Loop structure; Hepatitis B virus core particle

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2023 年 283 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF) is a highly infectious and lethal viral disease caused by the African swine fever virus (ASFV). The four prominent loop structures on the surface of the primary structural protein P72 are considered to be key protective epitopes. In this study, the four critical loops (ER1-4) of the ASFV p72 protein were indi-vidually fused to hepatitis B virus core particles (HBc) and self-assembled into nanoparticles to preserve the natural conformation of the loop structure and enhance its immunogenicity. Then, four recombinant proteins were obtained in E. coli expression system and monoclonal antibodies (mAbs) were developed and characterized. All 10 mAbs obtained were able to react with P72 protein and ASFV with potencies up to 1:204 800. Amino acids 250-274, 279-299 and 507-517 of the P72 protein were identified as linear epitopes and highly conserved. The mAb 4G8 showed the highest inhibition rate of 84% against ASFV positive sera. Importantly, neutralization experiments illustrated that mAb 4G8 has a 67% inhibition rate, indicating that its corresponding epitopes are potential candidates for ASFV vaccine. In conclusion, highly immunogenic nanoparticles of the ASFV P72 key loop were constructed to induce the production of highly effective mAbs and clarify their epitope information for the diagnosis and prevention of ASFV.

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