Integrated meta-omics reveals the regulatory landscape involved in lipid metabolism between pig breeds
文献类型： 外文期刊
作者： Sun, Jiajie 1 ; Xie, Fang 1 ; Wang, Jing 3 ; Luo, Junyi 1 ; Chen, Ting 1 ; Jiang, Qingyan 1 ; Xi, Qianyun 1 ; Liu, George E. 2 ; Zhang, Yongliang 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangdong Prov Key Lab Anim Nutr Control, Guangzhou 510642, Guangdong, Peoples R China
2.ARS, Anim Genom & Improvement Lab, USDA, BARC East, Beltsville, MD 20705 USA
3.Henan Acad Agr Sci, Inst Anim Husb & Vet Med, Zhengzhou 450002, Peoples R China
关键词： Animal model; Intermuscular fat; Single-cell RNA sequencing; Lipidomics; Metagenome
期刊名称：MICROBIOME （ 影响因子：15.5； 五年影响因子：19.4 ）
ISSN： 2049-2618
年卷期： 2024 年 12 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundDomesticated pigs serve as an ideal animal model for biomedical research and also provide the majority of meat for human consumption in China. Porcine intramuscular fat content associates with human health and diseases and is essential in pork quality. The molecular mechanisms controlling lipid metabolism and intramuscular fat accretion across tissues in pigs, and how these changes in response to pig breeds, remain largely unknown.ResultsWe surveyed the tissue-resident cell types of the porcine jejunum, colon, liver, and longissimus dorsi muscle between Lantang and Landrace breeds by single-cell RNA sequencing. Combining lipidomics and metagenomics approaches, we also characterized gene signatures and determined key discriminating markers of lipid digestibility, absorption, conversion, and deposition across tissues in two pig breeds. In Landrace, lean-meat swine mainly exhibited breed-specific advantages in lipid absorption and oxidation for energy supply in small and large intestinal epitheliums, nascent high-density lipoprotein synthesis for reverse cholesterol transport in enterocytes and hepatocytes, bile acid formation, and secretion for fat emulsification in hepatocytes, as well as intestinal-microbiota gene expression involved in lipid accumulation product. In Lantang, obese-meat swine showed a higher synthesis capacity of chylomicrons responsible for high serum triacylglycerol levels in small intestinal epitheliums, the predominant characteristics of lipid absorption in muscle tissue, and greater intramuscular adipcytogenesis potentials from muscular fibro-adipogenic progenitor subpopulation.ConclusionsThe findings enhanced our understanding of the cellular biology of lipid metabolism and opened new avenues to improve animal production and human diseases.D7xkEMpXE5aNHjWuhXhcDpVideo AbstractConclusionsThe findings enhanced our understanding of the cellular biology of lipid metabolism and opened new avenues to improve animal production and human diseases.D7xkEMpXE5aNHjWuhXhcDpVideo Abstract
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