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Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus

文献类型： 外文期刊

作者： Wang, Wei 1 ; Fan, Baochao 1 ; Zhang, Xuehan 1 ; Guo, Rongli 1 ; Zhao, Yongxiang 1 ; Zhou, Junming 1 ; Zhou, Jinzhu 1 ; Peng, Qi 1 ; Zhu, Mingjun 1 ; Li, Jizong 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Nanjing, Peoples R China

2.Zhejiang Sci Tech Univ, Shaoxing Acad Biomed, Shaoxing, Peoples R China

3.State Key Lab Cultivat Base Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, Nanjing, Peoples R China

4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Prevent & Control Avian Influenza & Other, Guangzhou, Peoples R China

关键词： porcine deltacoronavirus; colloidal gold immunochromatographic assay (GICA) strip; monoclonal antibodies; real-time PCR; cross-species transmission

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： Porcine deltacoronavirus (PDCoV) cause diarrhea and dehydration in newborn piglets and has the potential for cross-species transmission. Rapid and early diagnosis is important for preventing and controlling infectious disease. In this study, two monoclonal antibodies (mAbs) were generated, which could specifically recognize recombinant PDCoV nucleocapsid (rPDCoV-N) protein. A colloidal gold immunochromatographic assay (GICA) strip using these mAbs was developed to detect PDCoV antigens within 15 min. Results showed that the detection limit of the GICA strip developed in this study was 10(3) TCID50/ml for the suspension of virus-infected cell culture and 0.125 mu g/ml for rPDCoV-N protein, respectively. Besides, the GICA strip showed high specificity with no cross-reactivity with other porcine pathogenic viruses. Three hundred and twenty-five fecal samples were detected for PDCoV using the GICA strip and reverse transcription-quantitative real-time PCR (RT-qPCR). The coincidence rate of the GICA strip and RT-qPCR was 96.9%. The GICA strip had a diagnostic sensitivity of 88.9% and diagnostic specificity of 98.5%. The specific and efficient detection by the strip provides a convenient, rapid, easy to use and valuable diagnostic tool for PDCoV under laboratory and field conditions.

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