Differential physiological and proteomic responses of barley genotypes to sulfur availability
文献类型: 外文期刊
作者: Qiu, Cheng-Wei 1 ; Dawood, Muhammad 1 ; Zhao, Jing 1 ; Chen, Zhong-Hua 3 ; Wu, Feibo 1 ;
作者机构: 1.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Zijingang Campus, Hangzhou 310058, Peoples R China
2.Jilin Acad Agr Sci, Soybean Res Inst, Changchun 130033, Peoples R China
3.Western Sydney Univ, Sch Sci, Penrith, NSW 2751, Australia
关键词:
Sulfur deficiency;
期刊名称:PLANT GROWTH REGULATION ( 影响因子:3.9; 五年影响因子:3.8 )
ISSN: 0167-6903
年卷期: 2025 年
页码:
收录情况: SCI
摘要: Sulfur (S) is essential for plant growth, influencing protein synthesis and metabolic processes. However, S deficiency in agricultural soils is becoming more prevalent, negatively affecting crop yields and quality. In barley, where grain protein composition is crucial for malting and brewing, understanding the impact of S on nutrient balance and protein profiles is imperative. This study investigated the effects of S deficiency and supplementation on growth, mineral nutrition, amino acids, proteomics, and yield in two barley cultivars, ZheDa-9 (ZD-9) and Gairdner. Results show that S deprivation significantly reduced plant height, biomass, chlorophyll content, and grain yield, with Gairdner experiencing a 25.7% greater yield loss than ZD-9, indicating its higher sensitivity to S deficiency. Ionomic profiling revealed tissue-specific nutrient depletion, with Gairdner grains showing more pronounced reductions in S (-33.2%), N (-17.1%), Ca (-42.9%), Zn (-16.8%), and Cu (-33.0%). S-deficient grains exhibited altered amino acid profiles, characterized by N-rich amino acid accumulation and S-amino acid depletion. Genotype-specific patterns were observed: ZD-9 showed a 21.4% greater reduction in cysteine, while Gairdner exhibited a 25.4% decline in methionine, exacerbating N: S imbalance. Proteomic analysis revealed genotype-specific responses to S deficiency, with ZD-9 upregulating beta-amylase precursor and alpha-amylase inhibitor CMb, while gamma 3 hordein was downregulated compared to Gairdner. These findings highlight the importance of cultivar-specific sulfur management strategies, offering insights for optimizing S fertilization and selecting S-efficient barley genotypes to improve nutrient homeostasis, yield stability, and malting quality, particularly in low-S soils.
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