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Biological Characteristics and Molecular Mechanism of Procymidone Resistance in Stemphylium eturmiunum From Garlic

文献类型： 外文期刊

作者： Chen, Wen-chan 1 ; Wei, Ling-ling 1 ; Zheng, Huan-huan 1 ; Zhang, Peng-cheng 1 ; Wang, Bing-ran 1 ; Zhao, Wei-cheng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Pesticide, Nanjing 210095, Jiangsu, Peoples R China

2.Food Inspect & Testing Inst Henan Prov, Zhengzhou 450000, Henan, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

4.Heilongjiang Acad Agr Sci, Qiqihar Subacad, Qiqihar 161006, Heilongjiang, Peoples R China

关键词： decreased fitness; dicarboximides; garlic leaf blight; mutations at Se-HK1; Stemphylium eturmiunum

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2021 年 105 卷 7 期

页码：

收录情况： SCI

摘要： Garlic leaf blight caused by Stemphylium eturmiunum was first reported in Jiangsu Province in China. The dicarboximide fungicide (DCF) procymidone is reported to possess broad-spectrum action in inhibiting filamentous fungi and is widely used to control leaf disease of various plants. Of 41 Stemphylium eturmiunum isolates collected in this study from commercial garlic farms in Pizhou and Dafeng counties of Jiangsu Province, eight isolates were resistant to procymidone. The following three phenotypes were categorized according to in vitro responses to DCFs: sensitive, low resistance to iprodione and procymidone, and high resistance to all iprodione and procymidone. The fitness of all resistant isolates was decreased in accordance with data on mycelial growth, conidiation, and virulence. After treatment with 10 mu g/ml of procymidone for 4 h, mycelial intracellular glycerol concentrations of resistant isolates were significantly lower than those of sensitive isolates. Positive cross-resistance was observed between dicarboximides and phenylpyrroles, but there was no cross-resistance between dicarboximides and fluazinam or difenoconazole in the two resistant phenotypes. Nucleotide sequence alignment of two-component histidine kinase genes from sensitive and resistant isolates indicated that amino acid mutations were located at the histidine kinase, adenylyl cyclase, methyl-accepting chemotaxis protein and at the phosphatase domain of the N-terminal region and the response regulator domain of the C-terminal region. To our knowledge, this is the first report of DCF resistance in Stemphylium eturmiunum, and these findings will help establish a rational strategy to manage DCF-resistant populations of Stemphylium eturmiunum in the field.

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