A Genome-Wide Alternative Splicing Landscape Specifically Associated with Durable Rice Blast Resistance
文献类型： 外文期刊
作者： Liang, Dong 1 ; Yu, Junjie 1 ; Song, Tianqiao 1 ; Zhang, Rongsheng 1 ; Du, Yan 1 ; Yu, Mina 1 ; Cao, Huijuan 1 ; Pan, Xiayan 1 ; Qiao, Junqing 1 ; Liu, Youzhou 1 ; Qi, Zhongqiang 1 ; Liu, Yongfeng 1 ;
作者机构： 1.Jiangsu Acad Agr Sci JAAS, Nanjing 210014, Peoples R China
关键词： interaction; alternative splicing (AS) landscape; rice durable resistance; differentially alternatively spliced genes (DASGs)
期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）
ISSN：
年卷期： 2022 年 12 卷 10 期
页码：
收录情况： SCI
摘要： The rice blast, caused by the hemibiotrophic plant pathogen Magnaporthe oryzae, is a devastating disease that threatens rice crop production worldwide. The molecular interactions that underlie the rice-M. oryzae interaction have received much attention. However, genome-wide research focusing on alternative splicing (AS) has not been well-studied in rice-M. oryzae interactions. AS in plants leads to diverse proteomes without an expansion in gene numbers to regulate cellular processes during abiotic or biotic stress. The Pi21 gene negatively regulates rice resistance to M. oryzae infection, and thus the Pi21-RNAi silenced transgenic line (#241) exhibits partial but durable resistance. We compared the AS landscape in #241 and "Nipponbare" (Nip) during interacting with M. oryzae Guy11, and the alternative 3' splice-site (A3SS) is the most common AS type. GO enrichment analysis of #241-specific differentially alternatively spliced genes (DASGs) revealed that WRKY transcription factors (TFs), bHLH TFs, F-box protein with leucine rich repeats, AAA-type ATPase, and protein kinase were enriched in the GO terms "response to jasmonate acid (JA)" and "ethylene (ET)" at 24 h post-inoculation (hpi). At 48 hpi, one #241-specific DASG, ubiquitin gene (Os08g0295000), was predicted to be involved in endoplasmic reticulum (ER) stress. In silico analysis combined with PCR amplification confirmed that multiple isoforms are produced by Os08g0295000 and a skipped exon (SE) event results in isoform switching during interaction with M. oryzae. Protein-protein interaction (PPI) network analysis predicted that Os08g0295000-encoding proteins may interact with SNARE protein Q9LGF8 (Uniprot ID) to cooperatively regulate rice's response to M. oryzae. This study uncovered the AS profile of rice in response to M. oryzae, which will help to explore the linkage between AS and durable rice resistance.
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