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TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus

文献类型： 外文期刊

作者： Zhu, Xuejiao 1 ; Li, Fang 3 ; Fan, Baochao 1 ; Zhao, Yongxiang 1 ; Zhou, Junming 1 ; Wang, Dandan 1 ; Liu, Renqiang 3 ; Zhao, Dongming 3 ; Fan, Huiying 7 ; Li, Bin 1 ;

作者机构： 1.Minist Agr, Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China

3.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Natl African Swine Fever Para Reference Lab, Harbin, Peoples R China

4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing, Peoples R China

5.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

6.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Peoples R China

7.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

8.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China

关键词： ASFV; coagulation cascade; P72; factor 10; TRIM28

期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN： 0928-4249

年卷期： 2024 年 55 卷 1 期

页码：

收录情况： SCI

摘要： In 2018, African swine fever virus (ASFV) emerged in China, causing extremely serious economic losses to the domestic pig industry. Infection with ASFV can cause disseminated coagulation, leading to the consumption of platelets and coagulation factors and severe bleeding. However, the mechanism of virus-induced coagulation has yet to be established. In our study, ASFV downregulated the coagulation process, as detected by D-dimer (D2D) and Factor X (F10) expression in pigs challenged with ASFV HLJ/18. In vitro, ASFV infection increased Factor IX (F9) and Factor XII (F12) expression while downregulating F10 expression in porcine alveolar macrophages (PAMs). African swine fever virus induced both intrinsic and extrinsic coagulation cascades. In addition, several encoded proteins affect the expression of the crucial coagulation protein F10, and among the encoded proteins, p72 inhibits the activity and expression of F10. Proteomic analysis also revealed that p72 is involved in the coagulation cascade. p72 can interact with F10, and its inhibitory functional domains include amino acids 423-432 and amino acids 443-452. Finally, we found that F10 and p72 interact with tripartite motif-containing protein 28 (TRIM28). TRIM28 knockdown resulted in a decrease in F10 expression. Importantly, TRIM28 contributes to the reduction in F10 protein expression regulated by p72. Our findings revealed an inhibitory effect of the viral protein p72 on the ASFV infection-induced coagulation cascade and revealed a role of TRIM28 in reducing F10 expression, revealing a molecular mechanism of ASFV-associated coagulation.

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