文献类型： 外文期刊

作者： Yang, Ling ¹ ; Zhang, Qiongao ¹ ; Cui, Maosheng ² ; Zhang, Leying ¹ ; Ma, Yong ² ;

作者机构： 1.Hebei Univ Engn, Sch Life Sci & Food Engn, Handan, Peoples R China

2.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China

关键词： bisphenol A; embryo; oocyte; pig; tauroursodeoxycholic acid

期刊名称：ANIMAL SCIENCE JOURNAL （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 1344-3941

年卷期： 2024 年 95 卷 1 期

页码：

收录情况： SCI

摘要： Prolonged exposure of bisphenol A (BPA) has adverse effects on in vitro maturation (IVM) of oocytes, but treatment with tauroursodeoxycholic acid (TUDCA) can improve the IVM and development of embryos. The purpose of this study was to investigate the effects of BPA and both BPA and TUDCA on IVM and parthenogenetic development of embryos. The results showed that BPA treatment adverse effects on the cumulus expansion index, survival rate, polar body rate, mitochondrial distribution of the oocytes after maturation culture, and that it also decreased the cleavage rate and blastocyst rate of embryos after parthenogenetic develpoment. In addition, BPA treatment upregulated expression of genes related to endoplasmic reticulum stress and apoptosis and increased the intracellular reactive oxygen species (ROS) level, while it decreased expression of genes related to cumulus expansion. However, the supplementation of TUDCA relieved these adverse effects of BPA except polar body rate, blastocyst rate, and expression of BCL2 and PTGS1. In conclusion, the supplementation of TUDCA can partly attenuate the negative effects of BPA on IVM and parthenogenetic development of embryos, possibly by modification of the expression of genes related to endoplasmic reticulum stress, apoptosis and cumulus expansion, intracellular ROS level, and mitochondrial distribution.