Transcriptomic Analysis Revealed Candidate Genes Involved in Pseudomale Sperm Abnormalities in Chinese Tongue Sole (Cynoglossus semilaevis)
文献类型： 外文期刊
作者： Sun, Yuxuan 1 ; Li, Ming 1 ; Cui, Zhongkai 1 ; Zhang, Mengqian 1 ; Zhang, Tingting 1 ; Li, Lu 1 ; Wang, Na 1 ; Xu, Xiwen 1 ; Wei, Min 2 ; Xu, Wenteng 1 ;
作者机构： 1.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Pilot Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China
2.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang 222005, Peoples R China
关键词： Cynoglossus semilaevis; spermatogenesis; transcriptomic profile; FoxO signalling pathway; sex chromosome biased distribution
期刊名称：BIOLOGY-BASEL （ 影响因子：5.168； ）
ISSN：
年卷期： 2022 年 11 卷 12 期
页码：
收录情况： SCI
摘要： Chinese tongue sole (Cynoglossus semilaevis) has a ZZ/ZW sex determination system, but the genotypic female (ZW) can be sex-reversed into phenotypic males, namely, pseudomales. Pseudomale fish can produce only Z-type sperm but not W sperm. However, the molecular mechanism is unclear. To screen the key genes involved in pseudomale sperm abnormalities, we analysed the transcriptomic profiles of pseudomale and male sperm. In comparison to male sperm, 592 differentially expressed genes (DEGs) were identified in pseudomale sperm, including 499 upregulated and 93 downregulated genes. KEGG analysis indicated that the FoxO signalling pathway, especially the foxo3a and foxo6-like genes, may play an important role in spermatogenesis. The DEGs were mainly distributed on sex chromosomes, with 158 downregulated genes on the Z chromosome and 41 upregulated genes on the W chromosome. A specific area (14-15 M) on the Z chromosome was identified, which enriched eight DEGs inside the similar to 1 M region. In addition, there were five gene alleles on the sex chromosomes, which showed the opposite transcription pattern (upregulated for the W allele, downregulated for the Z allele). This study has provided valuable data for screening candidate genes involved in the pseudomale sperm abnormality.
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