Biological and Molecular Characterization of Two Closely Related Arepaviruses and Their Antagonistic Interaction in Nicotiana benthamiana
文献类型： 外文期刊
作者： Wang, Yaodi 1 ; Shen, Wentao 3 ; Dai, Zhaoji 1 ; Gou, Bei 1 ; Liu, Hongjun 1 ; Hu, Weiyao 1 ; Qin, Li 1 ; Li, Zengping 1 ; T 1 ;
作者机构： 1.Hainan Univ, Minist Educ, Key Lab Green Prevent & Control Trop Plant Dis &, Haikou, Hainan, Peoples R China
2.Hainan Univ, Coll Plant Protect, Haikou, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
关键词： infectious cDNA clone; pathological properties; viral accumulation; functional compatibility; virus-virus interactions; Potyviridae
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Previously, our group characterized two closely related viruses from Areca catechu, areca palm necrotic ringspot virus (ANRSV) and areca palm necrotic spindle-spot virus (ANSSV). These two viruses share a distinct genomic organization of leader proteases and represent the only two species of the newly established genus Arepavirus of the family Potyviridae. The biological features of the two viruses are largely unknown. In this study, we investigated the pathological properties, functional compatibility of viral elements, and interspecies interactions in the model plant, Nicotiana benthamiana. Using a newly obtained infectious clone of ANRSV, we showed that this virus induces more severe symptoms compared with ANSSV and that this is related to a rapid virus multiplication in planta. A series of hybrid viruses were constructed via the substitution of multiple elements in the ANRSV infectious clone with the counterparts of ANSSV. The replacement of either 5 '-UTR-HCPro1-HCPro2 or CI effectively supported replication and systemic infection of ANRSV, whereas individual substitution of P3-7K, 9K-NIa, and NIb-CP-3 '-UTR abolished viral infectivity. Finally, we demonstrated that ANRSV confers effective exclusion of ANSSV both in coinfection and super-infection assays. These results advance our understanding of fundamental aspects of these two distinct but closely related arepaviruses.
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