文献类型： 外文期刊
作者： Dai, Ge 1 ; Han, Kaikai 1 ; Huang, Xinmei 1 ; Zhang, Lijiao 1 ; Liu, Qingtao 1 ; Yang, Jing 1 ; Liu, Yuzhuo 1 ; Li, Yin 1 ; Zhao, Dongmin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China
3.Jiangsu Univ, Inst Life Sci, Zhenjiang, Jiangsu, Peoples R China
4.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China
关键词： Tembusu; Virus; HSP70; Viral life cycle
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）
ISSN： 0378-1135
年卷期： 2022 年 267 卷
页码：
收录情况： SCI
摘要： Tembusu virus (TMUV) is an avian-origined flavivirus that is prevalent in ducks and geese. TMUV causes reduced egg production and neurological problems, resulting in profound economic losses to the waterfowl industry. In the viral life cycle, cellular factors are required for viral entry, replication, assembly, release and so on. Heat shock protein 70 (HSP70) is reported to be involved in the replication of multiple viruses. In this study, we explored the roles of HSP70 in the TMUV life cycle. The results showed that TMUV infection induced HSP70 expression starting 12 h post-infection. An HSP70 inhibitor reduced TMUV viral RNA production and the number of virus particles, whereas an HSP70 activator enhanced the amount of viral RNA and virions that released from the cells. Further analysis revealed that HSP70 played important roles in the postentry stages of the TMUV life cycle, including viral replication, assembly and release. We also found that inhibition of HSP70 expression significantly reduced TMUV-induced apoptosis. Additionally, incubation of TMUV particles with an anti-HSP70 antibody significantly reduced viral infectivity, suggesting an association between HSP70 and TMUV particles. These results implicate HSP70 in the life cycle of TMUV, and therefore, targeting HSP70 may be a strategy for developing an anti-TMUV therapy.
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