您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner

文献类型： 外文期刊

作者： Zhu, Mingjun 1 ; Lv, Jiahuang 1 ; Wang, Wei 1 ; Guo, Rongli 1 ; Zhong, Chunyan 1 ; Antia, Avan 6 ; Zeng, Qiru 6 ; Li, Jizong 1 ; Liu, Qingtao 1 ; Zhou, Jinzhu 1 ; Zhu, Xuejiao 1 ; Fan, Baochao 1 ; Ding, Siyuan 6 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing, Jiangsu, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

4.Tibet Agr & Anim Husb Univ, Coll Anim Sci, Coll Vet Med, Nyingchi, Tibet, Peoples R China

5.Southwest Guizhou Vocat & Tech Coll Nationalities, Biol Engn Dept, Xingyi, Peoples R China

6.Washington Univ, Dept Mol Microbiol, Sch Med, St Louis, MO 63130 USA

期刊名称：PLOS BIOLOGY （ 影响因子：9.8； 五年影响因子：9.2 ）

ISSN： 1544-9173

年卷期： 2023 年 21 卷 3 期

页码：

收录情况： SCI

摘要： Coronaviruses (CoVs) comprise a group of important human and animal pathogens. Despite extensive research in the past 3 years, the host innate immune defense mechanisms against CoVs remain incompletely understood, limiting the development of effective antivirals and non-antibody-based therapeutics. Here, we performed an integrated transcriptomic analysis of porcine jejunal epithelial cells infected with porcine epidemic diarrhea virus (PEDV) and identified cytidine/uridine monophosphate kinase 2 (CMPK2) as a potential host restriction factor. CMPK2 exhibited modest antiviral activity against PEDV infection in multiple cell types. CMPK2 transcription was regulated by interferon-dependent and interferon regulatory factor 1 (IRF1)-dependent pathways post-PEDV infection. We demonstrated that 3 '-deoxy-3 ',4 '-didehydro-cytidine triphosphate (ddhCTP) catalysis by Viperin, another interferon-stimulated protein, was essential for CMPK2's antiviral activity. Both the classical catalytic domain and the newly identified antiviral key domain of CMPK2 played crucial roles in this process. Together, CMPK2, viperin, and ddhCTP suppressed the replication of several other CoVs of different genera through inhibition of the RNA-dependent RNA polymerase activities. Our results revealed a previously unknown function of CMPK2 as a restriction factor for CoVs, implying that CMPK2 might be an alternative target of interfering with the viral polymerase activity.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台