Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig
文献类型： 外文期刊
作者： Yi, JZ 1 ; Liu, MQ 2 ; Zhu, CZ 2 ; Zhang, Q 2 ; Sheng, ZT 2 ; Du, QY 2 ; Yan, WY 2 ; Zheng, ZX 2 ;
作者机构： 1.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China; Chinese Acad Agr Sci, Vet inst, Lanzhou 730046, Peoples R China; Zhejiang Acad Agr Sci, Virus Res Inst, Hangzhou 310021, Peoples R China
关键词： foot-and-mouth disease;FMDV serotype O;FMDV serotype A;VP1 protein;bivalent vaccine;guinea pig
期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）
ISSN：
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收录情况： SCI
摘要： In this study,two DNA fragments encoding amino acid (141-160)-(21-40))-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized.These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A.The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence.Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses.FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mug of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively.70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.
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