RNA interference taraetina VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice
文献类型： 外文期刊
作者： Chen, WZ 1 ; Yan, WY 2 ; Du, QY 2 ; Fei, LA 2 ; Liu, MQ 2 ; Ni, Z 2 ; Sheng, ZT 2 ; Zheng, ZX 2 ;
作者机构： 1.Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
2.Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China; Zhejiang Acad Agr Sci, Inst Biotechnol, Hangzhou 310021, Peoples R China
期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）
ISSN： 0022-538X
年卷期： 2004 年 78 卷 13 期
页码：
收录情况： SCI
摘要： RNA interference (RNAi) is a powerful tool to silence gene expression posttranscriptionally. In this study, we evaluated the antiviral potential of small interfering RNA (siRNA) targeting VP1 of foot-and-mouth disease virus (FMDV), which is essential during the life cycle of the virus and plays a key role in virus attachment to susceptible cells. We investigated in vivo the inhibitory effect of VP1-specific siRNAs on FMDV replication in BHK-21 cells and suckling mice, a commonly used small animal model. The results showed that transfection of siRNA-expressing plasmids gave an 80 to 90% reduction in the expression of FMDV VP1 in BHK-21 cells. Moreover, BHK-21 cells transiently transfected with siRNA-expressing plasmids were specifically resistant to FMDV infection when exposed to 100 50% tissue culture infective doses of virus, and the antiviral effects extended to almost 48 h postinfection. Furthermore, subcutaneous injection of siRNA-expressing plasmids in the neck made suckling mice significantly less susceptible to FMDV. In conclusion, our data suggests that RNAi may provide a viable therapeutic approach to treat FMDV infection.
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