Recombinant fusion protein and DNA vaccines against foot and mouth disease virus infection in guinea pig and swine
文献类型： 外文期刊
作者： Huang, HB 1 ; Yang, ZJ 2 ; Xu, QX 2 ; Sheng, ZT 2 ; Xie, Y 2 ; Yan, WY 2 ; You, YJ 2 ; Sun, LY 2 ; Zheng, ZX 2 ;
作者机构： 1.Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
2.Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China; Inst Vet Sci Shanghai, Shanghai, Peoples R China; Zhejiang Acad Agr Sci, Inst Virol, Hangzhou, Peoples R China; Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong
期刊名称：VIRAL IMMUNOLOGY （ 影响因子：2.257； 五年影响因子：1.882 ）
ISSN： 0882-8245
年卷期： 1999 年 12 卷 1 期
页码：
收录情况： SCI
摘要： In this study, we provide evidence that a recombinant fusion protein containing P-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.
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