Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos
文献类型: 外文期刊
作者: Cher, SL 1 ; Sha, ZX 1 ; Ye, HQ 1 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词: Differentiation;Embryonic stem cells;Lateolabrax japonicus;Medaka oryzias-latipes;Stem-cells;Mouse embryos;Fish;Chimeras;Differentiation;Transgenesis;Transmission;Expression;Cultures
期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Pluripotent embryonic stem (ES) cells provide an efficient approach for genome manipulation with many applications in marine biotechnology and development studies. To develop this technology, we have worked to derive fish ES cells for in vitro studies of embryo cell growth and differentiation and for the generation of transgenic fish. A pluripotent cell line, LJES1, was established from blastula-stage embryos of a cultured marine fish, Lateolabrax japonicus. The LJES1 cells were cultured in Dulbecco's modified Eagles medium (DMEM) supplemented with fetal bovine serum (FBS), marine fish serum, sea perch embryo extract, selenium, basic fibroblast growth factor (bFGF) and leukemia inhibitory factor. The ES-like cells were small and round or polygonal, and grew actively and stably in culture. The cells exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with all-trans retinoic acid, differentiation into various types of cells, including neuron-like cells, muscle cells and some unidentified cells were observed, suggesting that the LJES1 cells remained pluripotent in culture. Chromosome analysis revealed that LJES1 cells have a normal diploid karyotype with 2n = 48. Up to now, LJES1 cells have been continuously cultured for more than 150 days with more than 40 passages. High survival rate has been obtained after cryopreservation of cell cultures. GFP reporter gene were transferred into LJES1 cells and successfully expressed.
- 相关文献
作者其他论文 更多>>
-
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
作者:Chen, SL;Ren, GC;Sha, ZX;Hong, YH
关键词:turbot;Scophthalmus maximus;embryonic cell line;karyorype
-
Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization
作者:Ji, XS;Chen, SL;Tian, YS;Yu, GC;Sha, ZX;Xu, MY;Zhang, SC
关键词:cryopreservation;sperm;motility score;fertilization;sea perch;Lateolabrax japonicus
-
Derivation of a pluripotent embryonic cell line from red sea bream blastulas
作者:Chen, SL;Ye, HQ;Sha, ZX;Hong, Y
关键词:Chrysophrys major;Embryonic stem-like cells;In vitro differentiation;Medakafish oryzias-latipes;Stem-cells;Mouse embryos;Chimeras;Fish;Differentiation;Establishment;Zebrafish;Cultures;Transmission
-
Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation
作者:Chen, SL;Ren, GC;Sha, ZX;Shi, CY
关键词:
-
Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization
作者:Chen, SL;Ji, XS;Yu, GC;Tian, YS;Sha, ZX
关键词:Turbot;Scophthalmus maximus;Sperm;Cryopreservation;Motility;Salmo-gairdneri sperm;Atlantic salmon;Spermatozoa;Trout;Fertility;Storage;Fish;Milt;Carp