文献类型: 外文期刊
作者: Chen, SL 1 ; Ye, HQ 2 ; Sha, ZX 2 ; Hong, Y 2 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Shanghai Fisheries Univ, Shanghai 200090, Peoples R China; Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
关键词: Chrysophrys major;Embryonic stem-like cells;In vitro differentiation;Medakafish oryzias-latipes;Stem-cells;Mouse embryos;Chimeras;Fish;Differentiation;Establishment;Zebrafish;Cultures;Transmission
期刊名称:JOURNAL OF FISH BIOLOGY ( 影响因子:2.051; 五年影响因子:2.25 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A pluripotent cell line, sea bream embryonic stem-like cells (SBES1), was developed from blastula-stage embryos of the cultured red sea bream, Chrysophrys major. The SBES1 cells were cultivated in Dulbecco's modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor. They were small and round or polygonal, and grew actively and stabely in culture. The cells exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with all-trans retinoic acid, they differentiated into various types including neuron-like, neuroglia-like and muscle-like cells, suggesting that the SBES1 cells remained pluripotent in Culture. Chromosome analysis revealed that SBES1 cells had a normal diploid karyotype with 2n = 2st + 46t. At present, SBES1 cells have been cultured for > 180 days with more than 60 passages. High survival rate has been obtained after cryopreservation of cell cultures. This embryonic cell line may potentially be used for the production of transgenic red sea bream.
- 相关文献
作者其他论文 更多>>
-
Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers
作者:Liu, YG;Chen, SL;Li, J;Li, BF
关键词:Japanese flounder;Paralichthys olivaceus;ISSR marker;genetic diversity;genetic variation
-
Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus)
作者:Chen, SL;Wang, ZJ;Xu, MY;Gui, JF
关键词:Japanese flounder;Paralichthys olivaceus;Nramp;cDNA;expression
-
Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major)
作者:Chen, SL;Xu, MY;Ji, XS;Yu, GC;Liu, Y
关键词:
-
Analysis of genetic variation in selected stocks of hatchery flounder, Paralichthys olivaceus, using AFLP markers
作者:Liu, YG;Chen, SL;Li, BF;Wang, ZJ;Liu, ZJ
关键词:flounder;Paralichthys olivaceus;AFLP marker;genetic characteristics;genetic variation
-
Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers
作者:Liu, YG;Chen, SL;Li, BF
关键词:Japanese flounder;Paralichthys olivaceus;conservation;microsatellite;genetic structure
-
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
作者:Chen, SL;Ren, GC;Sha, ZX;Hong, YH
关键词:turbot;Scophthalmus maximus;embryonic cell line;karyorype
-
Isolation and characterization of polymorphic microsatellite loci from an EST-library of red sea bream (Chrysophrys major) and cross-species amplification
作者:Chen, SL;Liu, YG;Xu, MY;Li, J
关键词:cDNA;Chrysophrys major;cross-species amplification;microsatellite;red sea bream