Isolation and characterization of polymorphic microsatellite loci from an EST-library of red sea bream (Chrysophrys major) and cross-species amplification
文献类型: 外文期刊
作者: Chen, SL 1 ; Liu, YG 2 ; Xu, MY 2 ; Li, J 2 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Ocean Univ China, Div Life Sci & Technol, Qingdao 266003, Peoples R China
关键词: cDNA;Chrysophrys major;cross-species amplification;microsatellite;red sea bream
期刊名称:MOLECULAR ECOLOGY NOTES ( 影响因子:2.384; 五年影响因子:2.551 )
ISSN: 1471-8278
年卷期: 2005 年 5 卷 2 期
页码:
收录情况: SCI
摘要: Microsatellite markers have been developed from a complementary DNA (cDNA) library of red sea bream, Chrysophrys major. Twenty-eight microsatellites were selected for designing microsatellite primers, of which 11 gave working primer pairs. Observed and expected heterozygosities varied from 0.33 to 1.00 and from 0.38 to 0.83, respectively. Five additional fish species assessed for cross-species amplification revealed between one and six positive amplifications and between 0 and 6 polymorphic loci per species.
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