Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel)
文献类型: 外文期刊
作者: Li, J 1 ; Gao, DM 2 ; Wang, Q 2 ; Wang, JY 2 ; Wang, QY 2 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Ocean Univ China, Qingdao, Peoples R China
关键词: Vibrio anguillarum;immune response;antibody titre;relative percent survival;Japanese flounder
期刊名称:AQUACULTURE RESEARCH ( 影响因子:2.082; 五年影响因子:2.415 )
ISSN: 1355-557X
年卷期: 2005 年 36 卷 11 期
页码:
收录情况: SCI
摘要: Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel), were immunized by the intraperitoneal (i. p.) injection route with formalin-killed whole cells of Vibrio anguillarum that originated from a diseased fish. Fifty days later, a booster vaccination was given by the same route. Control fish were similarly treated with sterile phosphate-buffered saline. The efficacy of vaccination was evaluated based on protection against two bacterial challenges and immune responses (both specific and non-specific). The challenges were performed by i. p. injection with V. anguillarum or V. parahaemolyticus. The results indicated that the vaccinated fish showed higher non-specific immune activity than the unvaccinated fish. The effects of vaccinations on the phagocytic activity of phagocyte, bactericidal and lysozyme activities were notable, especially on bactericidal activity. Determined by ELISA, antiserum of vaccinated fish displayed high antibody titres. The vaccination conferred protection against V. anguillarum challenge (81.25-93.75% relative percentage survival (RPS)). The RPS was 46.15-53.85% against V. parahaemolyticus challenge, indicating some degree of cross-protective immunity.
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