Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper
文献类型： 外文期刊
作者： Tang, KX 1 ; Hu, QN 2 ; Sun, XF 2 ; Wan, BL 2 ; Qi, HX 2 ; Lu, XG 2 ;
作者机构： 1.Fudan Univ, Inst Genet, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China
2.Fudan Univ, Inst Genet, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China; Hubei Acad Agr Sci, Hybrid Rice Res Ctr, Wuhan 430064, Peoples R China
关键词： cotransformation;Oryza sativa L;particle bombardment;segregation analysis;snowdrop lectin gene (gna);transgenic rice
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
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收录情况： SCI
摘要： Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectahle marker hygromycin phosphotransferase gene (hpt), the reporter #bate#-glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. Thirty-five independent transgenic rice plants were regenerated from 177 bombarded calluses. Eighty-three percent of the transgenic plants contained all three genes, as revealed by Southern blot analysis. Western blot analysis revealed that 23 out of 29 gna-containing transgenic plants expressed Galanthus nivalis agglutinin (GNA) (79 percent) at various levels with the highest expression being approximately 0.5 percent of total soluble protein. Genetic analysis confirmed Mendelian segregation of all three transgenes (gna, hpt and gusA) in the R2 progeny. Amongst the R2 generation, two independent homozygous lines were identified that expressed all three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing the survival, overall fecundity of BPH, retarding development, and decreasing the feeding ofBPH. These BPH-resistant lines have been incorporated into a rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH.
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