文献类型： 外文期刊

作者： Yao, JH ¹ ; Zhao, XY ² ; Qi, HX ² ; Wan, BL ² ; Chen, F ² ; Sun, XF ² ; Yu, SQ ² ; Tang, KX ² ;

作者机构： 1.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn,Morgan Tan Int Ctr Life, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200433, Peoples R China

2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn,Morgan Tan Int Ctr Life, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200433, Peoples R China; Hubei Acad Agr Sci, Wuhan 430064, Hubei, Peoples R China; Shanghai Jiao Tong Univ, Sch Agr & Biol, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Plant Biotechnol Res Ctr, Shanghai 200030, Peoples R China

关键词： insect bioassay;Arisaema heterophyllum agglutinin;transformation;transgenic tobacco;peach potato aphid (Myzus persicae Sulzer)

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2004 年 84 卷 3 期

页码：

收录情况： SCI

摘要： Yao, J., Zhao, X., Qi, H., Wan, B., Chen, F., Sun, X., Yu, S. and Tang, K. 2004. Transgenic tobacco expressing an Arisaema heterophyllum agglutinin gene displays enhanced resistance to aphids. Can. J. Plant Sci. 84: 785-790. Tobacco leaf discs were transformed with a plasmid, pBIAHA, containing the selectable marker neomycin phosphotransferase gene (nptIl) and an Arisaema heterophyllum agglutinin gene (aha) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that multiple copies of the aha gene had integrated into the plant genome. Northern blot analysis revealed that the aha gene was expressed at various levels in the transgenic plants. Insect bioassay test showed that transgenic plants expressing multiple copies of the aha gene reduced the rate of population increase of the peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic tobacco plants expressing the aha gene display enhanced resistance to aphids.