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The pha2 gene cluster involved in Na+ resistance and adaption to alkaline pH in Sinorhizobium fredii RT19 encodes a monovalent cation/proton antiporter

文献类型： 外文期刊

作者： Yang, Lifu 1 ; Jiang, Juquan 2 ; Wei, Wei 2 ; Zhang, Bo 2 ; Wang, Lei 3 ; Yang, Susheng;

作者机构： 1.China Agr Univ, Dept Microbiol & Immunol, Coll Biol Sci, Beijing 100094, Peoples R China

2.China Agr Univ, Dept Microbiol & Immunol, Coll Biol Sci, Beijing 100094, Peoples R China; Minist Agr, Key Lab AgroMicrobial Resource & Applicat, Beijing, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Hainan, Peoples R China; Minist Agr, Key Lab Physiol Trop Crops, Hainan, Peoples R China

3.China Agr Univ, Dep

关键词： Sinorhizobium fredii;pha2;monovalent cation;proton antiporter;BACILLUS-SUBTILIS;SYSTEM;MRP;HOMEOSTASIS;ADAPTATION;CHOLATE;EFFLUX;ROLES;C-125

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sinorhizobium fredii RT19 can tolerate up to 0.6 M NaCl, whereas all its pha2-disrupted mutants, constructed by Tn5 mutagenesis, failed to grow in even the presence of 0.1 M NaCl. No growth difference was detected in pha2 mutants at a pH < 7.5 in the presence or absence of K+, but growth reduction was observed in the presence of K+ when pH > 7.5. The pha2 gene cluster was able to completely restore the growth of the pha2 mutants of S. fredii RT19 in 0.6 M NaCl. Measurement of monovalent cation intracellular content suggested that pha2 was involved in both Na+ (Li+) and K+ efflux. The pha2 mutants exhibited K+/H+, but no apparent Na+(Li+)/H+ antiporter activity in everted membrane vesicles. Taken together, these results indicated that the pha2 cluster of S. fredii RT19 encodes a monovalent cation/proton antiporter involved in resistance to Na+ and adaption to pH, which was very different from the pha1 cluster of Sinorhizobium meliloti, which encodes a K+/H+ antiporter.

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