Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae
文献类型： 外文期刊
作者： Wu, Jiahe 1 ; Luo, Xiaoli 1 ; Wang, Zhian 1 ; Tian, Yingchuan 4 ; Liang, Aihua 1 ; Sun, Yi 1 ;
作者机构： 1.Shanxi Acad Agr Sci, Inst Cotton Res & Biotechnol Res Ctr, Yuncheng 044000, Peoples R China
2.Shanxi Acad Agr Sci, Inst Cotton Res & Biotechnol Res Ctr, Taiyuan 030031, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Natl Key Lab Plant Genom, Beijing 100080, Peoples R China
4.Chines
关键词： Androctonus australis;bollworm control;cotton (transgenic);Gossypium hirsutum;scorpion toxin in cotton;PLANTS;INHIBITOR;PROTEIN;WEEVIL;VENOM
期刊名称：BIOTECHNOLOGY LETTERS （ 2020影响因子：2.461； 五年影响因子：2.457 ）
收录情况： SCI
摘要： A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44-98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.
- 相关文献
作者其他论文 更多>>
-
Introduction of Bacillus thuringiensis (Bt) gene does not reduce potassium use efficiency of Bt transgenic cotton (Gossypium hirsutum L.)
作者：Wang, Qianqian;Yan, Wei;Zhang, Yichi;Zhan, Manman;Li, Fangjun;Tian, Xiaoli;Luo, Xiaoli;Zhang, Anhong;Xiao, Juanli;Eneji, A. Egrinya
关键词：Biomass; Bt (Bacillus thuringiensis) gene; Cotton (Gossypium hirsutum L.); K uptake; K utilization index; Yield
-
RNA-Seq identification of candidate defense genes by analyzing Mythimna separata feeding-damage induced systemic resistance in balsas teosinte
作者：Sun, Yi;Wang, Chuangyun;Yang, Liyan;Gao, Jing;Zhang, Yurong;Tian, Jingyun
关键词：RNA-Seq; balsas teosinte; B73; bioassay; armyworm; insect-resistance
-
ABA alleviated soybean seedling stress exposed to UV-C radiation
作者：Yang, Liyan;Gao, Ning;Yang, Yashu;Zhang, Yurong;Gao, Jing;Sun, Yi
关键词：ultraviolet-C; ABA; active oxygen metabolism; flavonoids; soybean
-
Cotton plant defence against a fungal pathogen is enhanced by expanding BLADE-ON-PETIOLE1 expression beyond lateral-organ boundaries
作者：Zhang, Zhennan;Wang, Peng;Luo, Xiaoli;Yang, Chunlin;Tang, Ye;Wang, Zhian;Hu, Guang;Xia, Guixian;Wu, Jiahe;Ge, Xiaoyang
关键词：
-
Maize Genetic Transformation by Agrobacterium tumefaciens Assisted with Emery and Sonication Treatment
作者：Liang, Xue-lian;Chu, Kun;Nie, Xiao-xiong;Feng, Hua-jie;Han, Gui-li;Yi, Wen-bin;Wu, Qi-jing;Sun, Yi
关键词：Agrobacterium; Maize; Corundum powder; Ultrasonic; Germinated seeds; epsps gene
-
Double-Stranded RNAs High-Efficiently Protect Transgenic Potato from Leptinotarsa decemlineata by Disrupting Juvenile Hormone Biosynthesis
作者：Guo, Wenchao;Bai, Chao;Wang, Zhian;Wang, Peng;Fan, Qiang;Wang, Le;Pang, Jinhuan;Fu, Weidong;Tian, Yingchuan;Zhang, Guoliang;Wu, Jiahe;Guo, Wenchao;He, Jiang;Wang, Zhian;Wang, Peng;Luo, Xiaoli;Fan, Qiang;Mi, Xiaoxiao;Si, Huaijun;Bai, Chao;Fu, Weidong;Zhang, Guoliang
关键词：double-stranded RNAs; juvenile hormone acid methyltransferase (JHAMT); Leptinotarsa decemlineata Say.; pest control; RNA interference; transgenic potato
-
Expression of Foreign Genes Demonstrates the Effectiveness of Pollen-Mediated Transformation in Zea mays
作者：Yang, Liyan;Cui, Guimei;Wang, Yixue;Hao, Yaoshan;Du, Jianzhong;Wang, Changbiao;Zhang, Huanhuan;Wu, Shu-Biao;Sun, Yi;Yang, Liyan;Zhang, Hongmei;Wu, Shu-Biao;Sun, Yi
关键词：pollen-mediated transformation;sonication;eGfp;Cry1Ac;Zea mays
