中国水产科学研究院机构知识库

DEVELOPMENT OF MICROSATELLITE MARKERS IN KMERIA SEPTENTRIONALIS (MAGNOLIACEAE), AN ENDANGERED CHINESE TREE

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文献类型：外文期刊

作者： Lin, Yanfang ¹ ; Zeng, Liyan ³ ; Zhang, Qiwei ¹ ; Wang, Yaofang ¹ ; Tang, Shaoqing ¹ ;

作者机构： 1.Guangxi Normal Univ, Sch Life Sci, Minist Educ, Key Lab Ecol Rare & Endangered Species & Environm, Guilin 541004, Peoples R China

2.Chinese Acad Fishery Sci, Guangxi Key Lab Environm Engn Protect & Assessmen, Minist Agr, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

3.Chinese Acad Fishery Sci, Key Lab Marine & Estuarine Fisheries, Minist Agr, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： allele;microsatellite primer;polymorphic microsatellite marker: polymorphismSequence DataHQ888826: GenBank;EMBL;DDJB;nucleotid;HQ888827: GenBank;EMBL;DDJB;nucleotide sequence;HQ888828: GenBank;EMBL;DDJB;nucleotide sequence;HQ888829: GenBank;EMBL;DDJB;nucleotide sequence;HQ888830: GenBank;EMBL;DDJB;nucleotide sequence;HQ888831: GenBank;EMBL;DDJB;nucleotide sequence;HQ888832: GenBank;EMBL;DDJB;nucleotide sequence;HQ888833: GenBank;EMBL;DDJB;nucleotide sequence;HQ888834: GenBank;EMBL;DDJB;nucleotide sequence;HQ888835: GenBank;EMBL;DDJB;nucleotide sequence;HQ888836: GenBank;EMBL;DDJB;nucleotide sequence;HQ888837: GenBank;EMBL;DDJB;nucleotide sequence;HQ888838: GenBank;EMBL;DDJB;nucleotide sequence;HQ888839: GenBank;EMBL;DDJB;nucleotide sequence;genetic diversity;mating system;population genetic structure;gene fl ow

期刊名称：AMERICAN JOURNAL OF BOTANY （影响因子：3.844；五年影响因子：3.657 ）

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收录情况： SCI

摘要： Premise of the study: Microsatellite markers were developed in an endangered plant, Kmeria septentrionalis, to investigate its population genetic structure, gene flow, and mating systems.Methods and Results: Using the combined biotin capture method, 14 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from 3 to 15 across 60 individuals from two populations. The observed and expected heterozygosities ranged from 0.2692 to 0.9667 and from 0.3271 to 0.8881, respectively.Conclusions: These markers will facilitate further studies on the genetic diversity and mating systems of K. septentrionalis.

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