Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale, 1905 (Clupeiformes: Engraulidae) and cross-species amplification in Coilia mystus Linnaeus, 1758
文献类型: 外文期刊
作者: Ma, Chunyan 1 ; Cheng, Qiqun 1 ; Zhang, Qingyi 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Key Lab Marine & Estuarine Fisheries, Minist Agr, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
关键词: polymorphic microsatellite markerYangtze River: China;Asia;Palearctic region;heterozygosity;linkage disequilibrium;Hardy-Weinberg equilibrium;genetic structure;fishery management;cross-species amplification;genetic linkage map
期刊名称:ENVIRONMENTAL BIOLOGY OF FISHES ( 影响因子:1.844; 五年影响因子:1.839 )
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收录情况: SCI
摘要: In this study, twelve polymorphic microsatellite markers were developed for Coilia ectenes (synonym C. nasus). In a sample of 30 C. ectenes individuals in a Wuhan population from Yangtze River, a total of 78 alleles were detected, and the number of alleles per locus ranged from 3 to 12. The observed and expected heterozygosities ranged from 0.100 to 1.000 and from 0.098 to 0.899, respectively. Six loci significantly deviated from the Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.004), and no significant linkage disequilibrium between pairs of loci was found. The applicability of these markers in a closely related species, C. mystus, was evaluated by cross-species amplification. These microsatellite markers will be useful in studies on population genetics, conservation genetics, and fishery management and in the construction of genetic linkage maps of C. ectenes and C. mystus.
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