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The long-term effect of non-invasive sampling on the genetic diversity and growth performance of cultured Gymnocypris chilianensis (Cyprinidae) population: an evaluation based on 70 days

文献类型： 外文期刊

作者： Liu, Biyuan 1 ; Cheng, Qiqun 1 ; Lou, Zhongyu 3 ; Peng, Di 1 ; Qin, Yong 4 ; Song, Dan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Gansu Fisheries Res Inst, Lanzhou 730030, Peoples R China

4.Gansu Fishery Technol Promot Stn, Lanzhou 730030, Peoples R China

关键词： Non-invasive sampling; Gymnocypris chilianensis; molecular marker; Genetic diversity; growth performance

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.4； 五年影响因子：0.5 ）

ISSN： 0792-156X

年卷期： 2025 年 77 卷 2 期

页码：

收录情况： SCI

摘要： Non-invasive and non-lethal sampling methods are widely employed in conservation biology, but their long-term effects on sampled organisms remain understudied. To evaluate these impacts on fish and establish a scientific framework for ethical sampling practices, we conducted a dual investigation using the plateau-endemic species Gymnocypris chilianensis (Cyprinidae). First, genetic diversity in a cultured population was analyzed using non-invasive mucus sampling. Sequencing of mitochondrial Cytb (1098 bp) and D-loop (696 bp) regions revealed low genetic diversity, with Cytb showing six polymorphic sites, seven haplotypes (haplotype diversity H = 0.354; nucleotide diversity pi = 0.0004), and D-loop displaying 10 polymorphic sites, 9 haplotypes (H = 0.787; pi = 0.00315). The D-loop region demonstrated higher variability, supporting its utility as a marker for population genetic studies. Second, 180 individuals were divided into three groups, i.e., groups N (macus), Q (fin), and C (control), and monitored for 70 days to assess growth parameters (standard length, body weight), condition factor (K), and mortality. Generalized additive models (GAMs) revealed no significant differences in morphological indicators (p > 0.05) or mortality rates among groups, with a nonlinear standard length-weight regression relationship identified. Notably, all groups exhibited K values exceeding 200%, indicating excellent body condition. Comparative analyses confirmed minimal sequence divergence between non-invasive and traditional sampling methods, validating methodological reliability. This study provides the first long-term evaluation of non-invasive and non-lethal sampling impacts on fish growth, the results showed that non-invasive sampling had no significant impact on the growth or survival of fish, which was very important for future research and protection, combined with genetic diversity insights, offering critical guidelines for conserving vulnerable species like G. chilianensis in the Qinghai-Tibet Plateau.

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