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Isolation of a cinnamoyl CoA reductase gene involved in formation of stone cells in pear (Pyrus pyrifolia)

文献类型： 外文期刊

作者： Lu, Xiao-Peng 1 ; Liu, Yong-Zhong 1 ; An, Ji-Cui 1 ; Hu, Hong-Ju 2 ; Peng, Shu-Ang 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430209, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China

关键词： cDNA: complementary DNA;lignin: 9005-53-2;synthesis;regulation;cinnamoyl-CoA reductase: CCR;59929-39-4;EC 1.2.1.44;binding site;catalytic site;cinnamoyl CoA reductase-II;Genbank sequence data;fruit development;cell formation

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Stone cell which appears in most pear cultivars contributes badly to pear internal quality. Lignin is the important component of stone cells and its first biosynthetic but specific step is catalyzed by cinnamoyl CoA reductase (CCR). In the present study, a cDNA encoding CCR, named as PpCCR (GenBank accession no. GU138672), was isolated from pear (Pyrus pyrifolia) fruit. Its full length is 1,247 bp with a putative maximal open reading frame of 1,017 bp, encoding a protein of 339 amino acids. Sequence alignment revealed that the deduced protein contained a typical CCR catalytic site and NADP(H) binding site. Phylogenetic analysis showed that PpCCR belonged to dicot CCR class II in which most CCRs participated in the regulation of lignin biosynthesis. PpCCR transcript could be detected in leaves, flowers and fruits. In addition, PpCCR expression profiles during fruit development were compared between two cultivars with significant difference in stone cell content in the flesh. Although PpCCR expressed in a similar pattern between the two cultivars and their transcript level consisted strictly with the change of lignin content, its transcript level was always higher in the high-lignin content cultivar than in the low-lignin content cultivar during fruit development. Therefore, it could be suggested that PpCCR is involved in the regulation of lignin biosynthesis and plays an important role in stone cell formation in pear flesh.

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