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Identification of organic acid-related genes and their expression profiles in two pear (Pyrus pyrifolia) cultivars with difference in predominant acid type at fruit ripening stage

文献类型： 外文期刊

作者： Lu, Xiao-Peng 1 ; Liu, Yong-Zhong 1 ; Zhou, Gao-Feng 1 ; Wei, Qing-Jiang 1 ; Hu, Hong-Ju 3 ; Peng, Shu-Ang 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China

3.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430209, Peoples R China

关键词： malic acid: 6915-15-7;cytosolic aconitase;NADP-dependent malic enzyme: 9028-47-1;hydrogen ion-pyrophosphatase;hydrogen ion-ATPase A subunit;citric acid: 77-92-9;accumulation;citric synthase: 9027-96-7;EC 2.3.3.1;NADP-dependent isocitrate dehydrogenase: NADP-IDH;9028-48-2;EC 1.1.1.42;NAD-dependent malate dehydrogenase: NAD-MDH;9001-64-3;EC 1.1.1.37;fruit development;enzyme activity;fruit ripening stage;organic acid metabolism it is;organic acid transportation

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

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收录情况： SCI

摘要： 'Yandangxueli' is a pear cultivar with predominant citric acid in the ripe fruit, different from most of pear cultivars such as 'Gengtouqing' in which malic acid is the predominant acid type. It was found that 'Yandangxueli' accumulated citric acid for three times against that in 'Gengtouqing' at fruit ripening stage. To investigate the mechanism of citric acid accumulation in 'Yandangxueli', organic acids content, gene expression and enzyme activity were studied in both cultivars. Five genes, Pp:mtCs, Pp:cyAco, Pp:cyldh, Pp:mtMdh and Pp:cyMe which encoded citric synthase (CS), cytosolic aconitase (cyACO),NADP-dependent isocitrate dehydrogenase (NADP-IDH), NAD-dependent malate dehydrogenase (NAD-MDH) and NADP-dependent malic enzyme (NADP-ME) respectively, were identified from pear fruit Their expression profiles and the corresponding enzyme activities were determined throughout fruit development in both cultivars. Results from these enzymes indicated that there were no strict relationship between gene expression, enzyme activity and citric acid accumulation. Expression analysis for two Py:vVAtp genes encoding vacuolar H+-ATPase A subunit and one Py:vVpp gene encoding Vacuolar H+-pyrophosphatase showed that they were all with up-regulated expression at the later development stage of 'Yandangxueli' but with down-regulated expression in 'Gengtouqing'. Therefore, it is concluded that the different ability in citric acid transportation and storage might be involved in the high citric acid content in 'Yandangxueli'

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