Influences of the Interference of gamma-tubulin Gene Expression on the Morphology and Microtubules of Ciliate Euplotes eurystomus
文献类型： 外文期刊
作者： Sheng, Xin 1 ; Zeng, Hong 1 ; Zhang, Meng 1 ; Yun, Mi X. 1 ; Yin, Fei 3 ; Gu, Fu K. 1 ;
作者机构： 1.E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China
2.Fujian Normal Univ, Coll Life Sci, Fuzhou 350108, Peoples R China
3.Chinese Acad Fisheries Sci, E China Sea Fisheries Inst, Shanghai 200090, Peoples R China
关键词： dsRNA: double-stranded RNA;L4440-GTU: recombinant plasmid;cell division
期刊名称：ZOOLOGICAL SCIENCE （ 影响因子：0.931； 五年影响因子：1.036 ）
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收录情况： SCI
摘要： A recombinant plasmid L4440-GTU expressing.-tubulin dsRNA of the ciliate Euplotes eurystomus was constructed and transferred into Escherichia coli strain HT115. The resultant E. coli bacteria were fed on E. eurystomus to inhibit the ciliate's gamma-tubulin gene expression. As a result, the gamma-tubulin gene expression level was decreased, and this inactivation blocked cell division, which was lethal. In addition, the loss of the C-tubule from the nine-microtubule triplets in basal bodies, and the disappearance of some microtubules or mislocalization of some microtubule organization units in the subpellicular microtubule layers were also observed. These results indicate that the gamma-tubulin is not only important for the stability of the nine-microtubule triplets in basal bodies, but also necessary for the integrity of microtubule organization patterns in the subpellicular microtubule layer.
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