A Mitochondrial Model to Evaluate Endogenous Peroxidation in Litopenaeus vannamei: an in vitro Tool to Research Cellular Mechanisms of Antioxidants
文献类型： 外文期刊
作者： Liu, Xiao-Hua 1 ; Cao, Jun-Ming 1 ; Du, Zhen-Yu 4 ; Huang, Yan-Hua 1 ; Zhao, Hong-Xia 1 ; Zhu, Xuan 1 ; Zhou, Meng 1 ; La 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
3.Inst Anim Sci & Vet Med Wuhan, Wuhan 430065, Peoples R China
4.Natl Inst Nutr & Seafood Res NIFES, N-5005 Bergen, Norway
关键词： ascorbic acid: 62624-30-0;DNA helix;lipids: peroxidation;ferrous sulfate: 7720-78-7;reduced glutathione: GSH;70-18-8;antioxidant additive;aquatic feed;cellular oxidative state;research cellular mechanism;mitochondrial swelling rate
期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.287； 五年影响因子：0.593 ）
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收录情况： SCI
摘要： Mitochondrial lipid peroxidation is an important index in evaluating cellular oxidative states in biomedical research. In order to develop a method to study lipid peroxidative process and cellular mechanisms of antioxidants in aquatic animals, we established a mitochondrial model to evaluate endogenous peroxidation in Litopenaeus vannamei. The mitochondrial fraction was isolated from the hepatopancreas and mitochondrial lipid peroxidation was induced by ascorbic acid/FeSO4 or NADH to evaluate thiobarbituric acid reactive substances (TBARS), the mitochondrial swelling rate, and the unwinding rate of the DNA helix. Induced mitochondrial peroxidation was significantly inhibited by low doses of exogenous reduced glutathione (GSH), which is used as an antioxidant in humans and other mammals but is not commonly applied in aquaculture. Our in vitro model can be an effective tool for studying cellular mechanisms of antioxidants.
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