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Effect of Long-Term Administration of Dietary beta-Glucan on the Expression of Immune-Related Genes in Litopenaeus vannamei

文献类型: 外文期刊

作者: Zhao, Hong-Xia 1 ; Cao, Jun-Ming 1 ; Wang, An-Li 2 ; Huang, Yan-Hua 1 ; Li, Guo-Li 1 ; Lan, Han-Bing 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

2.S China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangzhou 510631, Guangdong, Peoples R China

关键词: Litopenaeus vannamei;beta-1,3-glucan;gene expression;immune-related genes

期刊名称:ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH ( 影响因子:0.287; 五年影响因子:0.593 )

ISSN: 0792-156X

年卷期: 2013 年 65 卷

页码:

收录情况: SCI

摘要: Expression of immune-related genes in muscle was evaluated in a 84-day feeding trial in white shrimp, Litopenaeus vannamei, fed diets containing 0, 250, 500, or 1000 mg/kg beta-1,3-glucan from Laminaria digitata. The transcript abundance of hemocyanin, lipopolysaccharide/b-glucan binding protein (LGBP), penaeidin-3, prophenoloxidase (proPO), serine proteinase, and cytosolic manganese superoxide dismutase (cMnSOD) was quantified by real-time PCR. The dietary inclusion of beta-1,3-glucan significantly affected the mRNA expression of all six genes. LGBP, cMnSOD, and hemocyanin were significantly up-regulated by day 14, serine proteinase peaked on day 28, and penaeidin-3 and ProPO peaked on day 56. The dosage of beta-1,3-glucan also affected mRNA expression. cMnSOD was significantly higher in shrimp fed the diets containing 250 or 500 mg/kg beta-1,3-glucan than in those fed the control. ProPO and penaeidin-3 were significantly higher in shrimp fed the diet containing 500 mg/kg beta-1,3-glucan than in those fed the control. LGBP, serine proteinase, and hemocyanin were significantly higher in shrimp fed the 500 or 1000 mg/kg than in those fed the control. Thus, feeding diets with 250, 500, or 1000 mg beta-1,3-glucan/kg dry diet can help improve the expression of immune-related genes in L. vannamei.

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