文献类型: 外文期刊
作者: Yi, Yang 1 ; Zhang, Ming-Wei 1 ; Liao, Sen-Tai 1 ; Zhang, Rui-Fen 1 ; Deng, Yuan-Yuan 1 ; Wei, Zhen-Cheng 1 ; Tang, Xi 1 ;
作者机构: 1.Guangdong Acad Agr Sci, Key Lab Funct Food, Minist Agr, Biotech Res Inst, Guangzhou 510610, Guangdong, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, People
关键词: Longan pulp;Polysaccharide;Structural feature;Immunomodulatory activity
期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: An aqueous extract of polysaccharides from longan pulp was chromatographed on a DEAE anion-exchange column to yield four fractions (LP1-IV). Immunomodulatory activities of these polysaccharides were also evaluated in vitro. The purified products, neutral polysaccharide LPI, polysaccharide-protein complex LPI1 and acidic polysaccharides LPI1I and LPIV, exhibited conspicuous differences in their monosaccharide composition, molecular mass and glycosidic linkages. Except for LPI, the other three significantly stimulated lymphocyte proliferation in the dose range of 100-400 μg/mL compared with the normal control (P<0.05), and might electively stimulate B cells, but not T cells. Furthermore, their stimulations on normal/lipopolysaccharide-induced proliferation and depressions on concanavalin A-induced proliferation could be ordered as LPIII > LPIV > LPII > LPI. All the fractions had the optimal dose of 100 μg/mL on enhancing macrophage phagocytosis. Among them, LPII had the considerable yield and activity for exploiting as a potential immunoadjuvant.
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