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Fed-batch fermentation of recombinant Citrobacter freundii with expression of a violacein-synthesizing gene cluster for efficient violacein production from glycerol

文献类型： 外文期刊

作者： Yang, Cheng 1 ; Jiang, Peixia 1 ; Xiao, Su 1 ; Zhang, Chong 1 ; Lou, Kai 2 ; Xing, Xin-Hui 1 ;

作者机构： 1.Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, Beijing 100084, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830000, Peoples R China

关键词： Citrobacter freundii;Fed-batch culture;Fermentation;Heterogeneous biocatalysis;Production kinetics;Violacein

期刊名称：BIOCHEMICAL ENGINEERING JOURNAL （ 影响因子：3.978； 五年影响因子：4.084 ）

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收录情况： SCI

摘要： The extensive prospects of violacein in the pharmaceutical industry have attracted increasing interest. However, the fermentation levels of violacein are currently inadequate to meet the demands of industrial production. This study was undertaken to develop an efficient process for the production of violacein by recombinant Citrobacter freundii. The effects of dissolved oxygen (DO) and pH on cell growth and violacein production in batch cultures were investigated first. When the DO and pH of the medium were controlled at around 25% and 7.0, respectively, the biomass and concentration of violacein were maximized. Based on the consumption of nutrients in the medium observed during batch culture, a fed-batch fermentation strategy with controlled DO and pH was implemented. By continuously feeding glycerol, NH4Cl, and L-tryptophan at a constant feeding rate of 16mL h~(-1), the final concentration of violacein reached 4.13g L~(-1), which was 4.09-fold higher than the corresponding batch culture, and the maximal dry cell weight (DCW) and average violacein productivity obtained for the fed-batch culture were 3.34 g DCWL~(-1) and 82.6 mgL~(-1) h~(-1), respectively. To date, this is the first report on the efficient production of violacein by genetically engineered strains in a fermentor.

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[1]Production of violet pigment by a newly isolated psychrotrophic bacterium from a glacier in Xinjiang, China. Lu, Yuan,Wang, Liyan,Xue, Yuan,Zhang, Chong,Xing, Xin-Hui,Lou, Kai,Zhang, Zhidong,Li, Yong,Zhang, Guifeng,Bi, Jingxiu,Su, Zhiguo.

[2]Optimization of culture conditions for violacein production by a new strain of Duganella sp B2. Jiang, Peixia,Lu, Yuan,Xing, Xin-Hui,Wang, Haisheng,Ruan, Zhiyong,Jiang, Ruibo,Lou, Kai,Wei, Dong. 2009

[3]Reconstruction of the violacein biosynthetic pathway from Duganella sp B2 in different heterologous hosts. Jiang, Pei-xia,Zhang, Chong,Xing, Xin-Hui,Wang, Hai-sheng,Lou, Kai. 2010

[4]Pathway redesign for deoxyviolacein biosynthesis in Citrobacter freundii and characterization of this pigment. Jiang, Pei-xia,Xiao, Su,Fang, Ming-yue,Zhang, Rui-ping,Xing, Xin-Hui,Jiang, Pei-xia,Wang, Hai-sheng,He, Shu-ying,Lou, Kai. 2012

[5]High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Fang, Ming-Yue,Zhang, Chong,Xing, Xin-Hui,Yang, Song,Cui, Jin-Yu,Jiang, Pei-Xia,Lou, Kai,Wachi, Masaaki. 2015

[6]A high-throughput screening method for identifying lycopene-overproducing E. coli strain based on an antioxidant capacity assay. Xu, Xian,Jin, Weiyue,Xu, Qing,Li, Shuang,Huang, He,Jiang, Ling,Zhang, Zhidong,Huang, He.

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