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Molecular cloning and expression analysis of CmMlo1 in melon

文献类型： 外文期刊

作者： Cheng, Hong 1 ; Kun, Weiping 1 ; Liu, Dongshun 1 ; Su, Yongquan 1 ; He, Qiwei 2 ;

作者机构： 1.Gansu Acad Agr Sci, Inst Vegetable, Lanzhou 730070, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetable, Jinan 250100, Peoples R China

关键词： Cloning;Expression;Melon;Mlo

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Mlo gene encodes an important transmembrane protein that is involved in biotic/abiotic stresses. Using the method of homologous, we cloned a Mlo gene from melon, named CmMlo1. The gene is 1551 bp in length, encoding 516 amino acids; it has seven-transmembrane domain topology and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMlo1-GFP is localized to the plasma membrane. RT-PCR results indicated that CmMlo1 is mainly expressed in melon cotyledon and flower, with a tissue-specific distribution manner. CmMlo1 expression is not obvious under powdery mildew stress, but under cadmium stress, its expression was significantly up-regulated, indicating that CmMlo1 is possibly involved in abiotic stress.

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