文献类型： 外文期刊

作者： Yin, Jing ¹ ; Ren, Chun-Lin ¹ ; Zhan, Ya-Guang ¹ ; Li, Chun-Xiao ¹ ; Xiao, Jia-Lei ² ; Qiu, Wei ¹ ; Li, Xin-Yu ¹ ; Peng, ¹ ;

作者机构： 1.NE Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China

2.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

3.NE Forestry Univ, Maoershan Expt Forestry Ctr, Harbin 150040, Peoples R China

关键词： Birch (Betula platyphylla suk.);Betulin;Oleanolic acid;Biosynthesis;Gene expression

期刊名称：MOLECULAR BIOLOGY REPORTS （ 2020影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Betulin and oleanolic acids (pentacyclic triterpenoid secondary metabolites) have broad pharmacological activities and can be potentially used for the development of anti-cancer and anti-AIDS drugs. In this study, we detected the accumulation and the distribution characteristics of betulin and oleanolic acid in various organs of white birch at different ages. We also determined the expression of 4 OSC genes (LUS, beta-AS, CAS1 and CAS2) involved in the triterpenoid synthesis pathways by real time RT-PCR. The result showed that the 1-year old birch can synthesize betulin and oleanolic acid. In addition, betulin and oleanolic acids were mainly distributed in the bark, while the content in the root skin and leaf was very low. The content of betulin and oleanolic acid in birch varied in different seasons. The content of betulin and oleanolic acid and their corresponding LUS and beta-AS gene expression were very low in 1-year old birch. With increasing age of birch, betulin content was increased, while oleanolic acid was decreased. Similar changes were also observed for their corresponding synthesis genes LUS and beta-AS. In the leaf of 1-year old plant, the highest expression of CAS1 and CAS2 occurred at end of September, while expression of LUS and the beta-AS was low from June to October. In the stem skin, high expression of beta-AS and the LUS genes occurred from the end of July to September. In the root, high expression of the beta-AS gene was observed at the end of October. These results indicated that triterpenoid gene expression was similar to the triterpene accumulation. Expression of LUS gene and beta-AS gene in birch with different ages were corresponding to the betulinic and oleanolic acid accumulation. Expression of CAS1 and CAS2 genes were elevated with increasing age of birch. This study provides molecular mechanisms of triterpenes synthesis in birch plants.